Journal article
Inhibition of hepatic gluconeogenesis and enhanced glucose uptake contribute to the development of hypoglycemia in mice bearing interleukin-1β-secreting tumor
Endocrinology (Philadelphia), Vol.145(11), pp.5150-5156
2004
DOI: 10.1210/en.2004-0323
PMID: 15297440
Abstract
Mice bearing IL-1β-secreting tumor were used to study the chronic effect of IL-1β on glucose metabolism. Mice were injected with syngeneic tumor cells transduced with the human IL-1β gene. Serum IL-1β levels increased exponentially with time. Secretion of IL-1β from the developed tumors was associated with decreased food consumption, reduced body weight, and reduced blood glucose levels. Body composition analysis revealed that IL-1β caused a significant loss in fat tissue without affecting lean body mass and water content. Hepatic phosphoenolpyruvate carboxykinase and glucose-6-phosphatase activities and mRNA levels of these enzymes were reduced, and 2-deoxy-glucose uptake by peripheral tissues was enhanced. mRNA levels of glucose transporters (Gluts) in the liver were determined by real-time PCR analysis. Glut-3 mRNA levels were up-regulated by IL-1β. Glut-1 and Glut-4 mRNA levels in IL-1β mice were similar to mRNA levels in pair-fed mice bearing nonsecreting tumor. mRNA level of Glut-2, the major Glut of the liver, was down-regulated by IL-1β. We concluded that both decreased glucose production by the liver and enhanced glucose disposal lead to the development of hypoglycemic in mice bearing IL-1β-secreting tumor. The observed changes in expression of hepatic Gluts that are not dependent on insulin may contribute to the increased glucose uptake.
Details
- Title: Subtitle
- Inhibition of hepatic gluconeogenesis and enhanced glucose uptake contribute to the development of hypoglycemia in mice bearing interleukin-1β-secreting tumor
- Creators
- Shulamit Metzger - Hadassah Academic CollegeSamir Nusair - Hadassah Academic CollegeDavid Planer - Hadassah Academic CollegeVarda Barash - Hadassah Medical CenterOrit Pappo - Hadassah Medical CenterJoel Shilyansky - Medical College of WisconsinTova CHAJEK-SHAUL - Hadassah Academic College
- Resource Type
- Journal article
- Publication Details
- Endocrinology (Philadelphia), Vol.145(11), pp.5150-5156
- DOI
- 10.1210/en.2004-0323
- PMID
- 15297440
- NLM abbreviation
- Endocrinology
- ISSN
- 0013-7227
- eISSN
- 1945-7170
- Publisher
- Endocrine Society
- Language
- English
- Date published
- 2004
- Academic Unit
- Stead Family Department of Pediatrics; Surgery
- Record Identifier
- 9984322822802771
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