Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus

Maria A Nagel; Stephanie F James; Igor Traktinskiy; Ann Wyborny; Alexander Choe; April Rempel; Nicholas L Baird; Don Gilden

doi:10.1128/JVI.01945-14

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Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus

Journal article

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Peer reviewed

Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus

Maria A Nagel, Stephanie F James, Igor Traktinskiy, Ann Wyborny, Alexander Choe, April Rempel, Nicholas L Baird and Don Gilden

Journal of virology, Vol.88(19), pp.11634-11637

10/01/2014

DOI: 10.1128/JVI.01945-14

PMCID: PMC4178816

PMID: 25056900

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Abstract

In varicella-zoster virus (VZV)-infected primary human brain vascular adventitial fibroblasts (BRAFs), levels of beta interferon (IFN-β,) STAT1, and STAT2 transcripts as well as STAT1 and STAT2 protein were decreased. IFN-α transcript levels were increased but not secreted IFN-α protein levels. Compared to IFN-α-treated control results, in VZV-infected BRAFs, phosphorylated STAT1 did not translocate to the nucleus, resulting in impaired downstream expression of interferon-inducible antiviral Mx1. Overall, VZV interference with the type I interferon pathway may promote virus persistence in cerebral arteries.

Adventitia - blood supply

Adventitia - metabolism

Adventitia - pathology

Adventitia - virology

Blood Vessels - metabolism

Blood Vessels - pathology

Blood Vessels - virology

Brain - blood supply

Brain - metabolism

Brain - pathology

Brain - virology

Fibroblasts - metabolism

Fibroblasts - pathology

Fibroblasts - virology

Gene Expression Regulation

Herpesvirus 3, Human - genetics

Herpesvirus 3, Human - metabolism

Host-Pathogen Interactions

Humans

Interferon-alpha - genetics

Interferon-alpha - metabolism

Interferon-beta - genetics

Interferon-beta - metabolism

Myxovirus Resistance Proteins - antagonists & inhibitors

Myxovirus Resistance Proteins - genetics

Myxovirus Resistance Proteins - metabolism

Phosphorylation

Primary Cell Culture

Protein Transport

Signal Transduction

STAT1 Transcription Factor - genetics

STAT1 Transcription Factor - metabolism

STAT2 Transcription Factor - genetics

STAT2 Transcription Factor - metabolism

Details

Title: Subtitle: Inhibition of phosphorylated-STAT1 nuclear translocation and antiviral protein expression in human brain vascular adventitial fibroblasts infected with varicella-zoster virus
Creators: Maria A Nagel - University of Colorado Denver
Stephanie F James - University of Colorado Denver
Igor Traktinskiy - University of Colorado Denver
Ann Wyborny - University of Colorado Denver
Alexander Choe - University of Colorado Denver
April Rempel - University of Colorado Denver
Nicholas L Baird - University of Colorado Denver
Don Gilden - University of Colorado Denver
Resource Type: Journal article
Publication Details: Journal of virology, Vol.88(19), pp.11634-11637
DOI: 10.1128/JVI.01945-14
PMID: 25056900
PMCID: PMC4178816
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Grant note: R01 AG006127 / NIA NIH HHS AG006127 / NIA NIH HHS K08 NS067070 / NINDS NIH HHS UL1 TR001082 / NCATS NIH HHS NS 067070 / NINDS NIH HHS AG032958 / NIA NIH HHS P01 AG032958 / NIA NIH HHS T32 NS007321 / NINDS NIH HHS R37 AG006127 / NIA NIH HHS NS007321 / NINDS NIH HHS
Language: English
Date published: 10/01/2014
Academic Unit: Neurology
Record Identifier: 9984827337302771

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