Journal article
Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin
Journal of cataract and refractive surgery, Vol.20(5), pp.513-516
09/01/1994
DOI: 10.1016/S0886-3350(13)80230-1
PMID: 7996406
Abstract
We investigated the effect of an antitransferrin receptor immunotoxin (454A12-rRA) on proliferating human and baboon lens epithelium in vitro. Human and baboon lens epithelial cells grown in modified TC-199 medium at 35 degrees Celsius in 7% C02 were seeded in 24 well plates at a density of 17,500 cells/ml to 40,000 cells/ml. The cells were exposed to various concentrations of 454A12-rRA for seven days. The sensitivity of proliferating human lens epithelium to 454A12-rRA was dependent on the dose, with a 60% to 70% reduction in cell counts at immunotoxin concentrations of 100 ng/ml and above. The immunotoxin had no significant effect on baboon lens epithelium in vitro, which suggests that it is specific for human tissue. By preventing the proliferation of human lens epithelial cells, immunotoxin 454A12-rRA may be useful in the management of posterior capsule opacification after planned extracapsular cataract surgery.
Details
- Title: Subtitle
- Inhibition of proliferating lens epithelium with antitransferrin receptor immunotoxin
- Creators
- Kenneth M. Goins - University of Chicago Medical CenterJulio R. Ortiz - Duke UniversitySam F.A. Fulcher - Duke UniversityJames T. Handa - Duke UniversityGlenn J. Jaffe - Duke UniversityGary N. Foulks - Duke UniversityL. Michael Cobo - Duke University
- Resource Type
- Journal article
- Publication Details
- Journal of cataract and refractive surgery, Vol.20(5), pp.513-516
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0886-3350(13)80230-1
- PMID
- 7996406
- ISSN
- 0886-3350
- eISSN
- 1873-4502
- Number of pages
- 4
- Language
- English
- Date published
- 09/01/1994
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984806606302771
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