Initial T Cell Receptor Transgenic Cell Precursor Frequency Dictates Critical Aspects of the CD8 + T Cell Response to Infection

Vladimir P Badovinac; Jodie S Haring; John T Harty

doi:10.1016/j.immuni.2007.04.013

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Initial T Cell Receptor Transgenic Cell Precursor Frequency Dictates Critical Aspects of the CD8 + T Cell Response to Infection

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Initial T Cell Receptor Transgenic Cell Precursor Frequency Dictates Critical Aspects of the CD8 + T Cell Response to Infection

Vladimir P Badovinac, Jodie S Haring and John T Harty

Immunity (Cambridge, Mass.), Vol.26(6), pp.827-841

2007

DOI: 10.1016/j.immuni.2007.04.013

PMID: 17555991

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Abstract

Adoptive-transfer experiments with relatively large input numbers (∼10 6) of T cell receptor-transgenic (TCR-tg) T cells are widely used to model endogenous T cell responses to infection or immunization. We show that input numbers of naive TCR-tg T cells sufficient to squelch the endogenous response to the same epitope substantially alter the kinetics, proliferative expansion, phenotype, and efficiency of memory generation by the TCR-tg T cells in response to infection. Thus, responses from nonphysiologic input numbers of TCR-tg T cells fail to accurately mimic the endogenous T cell response. Importantly, seeding as few as ∼10–50 TCR-tg T cells, which constitute a fraction of the endogenous repertoire, allowed vigorous proliferation and analysis of TCR-tg cells after infection in a scenario representing normal physiology for any individual TCR. These data strongly suggest that modeling the endogenous T cell response with TCR-tg cells will require every effort to approximate the endogenous precursor frequency.

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Details

Title: Subtitle: Initial T Cell Receptor Transgenic Cell Precursor Frequency Dictates Critical Aspects of the CD8 + T Cell Response to Infection
Creators: Vladimir P Badovinac - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Jodie S Haring - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
John T Harty - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Immunity (Cambridge, Mass.), Vol.26(6), pp.827-841
Publisher: Elsevier Inc
DOI: 10.1016/j.immuni.2007.04.013
PMID: 17555991
ISSN: 1074-7613
eISSN: 1097-4180
Language: English
Date published: 2007
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984046935202771

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