Journal article
Initial characterization of the organophosphate acid anhydrase activity of the chicken, Gallus domesticus
Comparative biochemistry and physiology. C, Comparative pharmacology, Vol.102(2), pp.253-265
1992
DOI: 10.1016/0742-8413(92)90109-K
Abstract
1. Supernatant solutions from kidney and liver homogenates of the chicken, Gallus domesticus, were found to hydrolyze the organophosphate (OP) compound diisopropylfluorophosphate (DFP). The activity on DFP as substrate was heat-inactivated and was characterized for temperature and pH optima, enzyme kinetics, and requirements for manganous ion. 2. Gel column chromatography indicated that the DFPase in both tissues is in the range of 82,100 to 93,300 D. This activity is strongly inhibited by N,N'-diisopropylphosphorodia-midofluoridate (mipafox). 3. The chicken has organophosphate acid (OPA) anhydrase activity comparable to other eucaryotic sources in its ability to hydrolyze DFP. Although birds may not have paraoxonase activity comparable to mammalian species, they do not differ significantly in the ability to hydrolyze DFP and probably related compounds.
Details
- Title: Subtitle
- Initial characterization of the organophosphate acid anhydrase activity of the chicken, Gallus domesticus
- Creators
- Brian D Westra - Western Washington UniversityWayne G Landis - Western Washington University
- Resource Type
- Journal article
- Publication Details
- Comparative biochemistry and physiology. C, Comparative pharmacology, Vol.102(2), pp.253-265
- DOI
- 10.1016/0742-8413(92)90109-K
- ISSN
- 0306-4492
- Language
- English
- Date published
- 1992
- Academic Unit
- Humanities and Social Sciences/Scholarly Impact
- Record Identifier
- 9983992020602771
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