Journal article
Initiation of translation at CUG, GUG, and ACG codons in mammalian cells
Gene, Vol.91(2), pp.173-178
1990
DOI: 10.1016/0378-1119(90)90085-6
PMID: 2170233
Abstract
Site-directed mutants of the ACG start codon of the C′ protein encoded in the polycistronic Sendai virus
P/C mRNA revealed that CUG, GUG, and ACG codons initiated translation rather efficiently (10–30% of the AUG initiation) in COS-1 host cells. In addition, AUA and AUU codons initiated translation at about 5% efficiency, while UUG did not initiate translation. The sequence context of these start codons (purine residues at −3 and +4) was crucial in their recognition by the ribosome. The location of the non-AUG codons in the
P/C mRNA did not play a role in its recognition by ribosomes. By using CUG, the most efficient non-AUG start codon, instead of the original ACG codon and inserting an additional upstream CUG codon in the
P/C mRNA, the amount of the C′ protein was increased and a novel protein was synthesized. Syntheses of an increased level of C′ and the novel protein did not affect downstream initiations of the P and C proteins, suggesting that more ribosomes bind the mRNA than are actually utilized for initiation of translation.
Details
- Title: Subtitle
- Initiation of translation at CUG, GUG, and ACG codons in mammalian cells
- Creators
- Haider Mehdi - Rush University Medical CenterEtsuro Ono - Rush University Medical CenterKailash C. Gupta - Rush University Medical Center
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.91(2), pp.173-178
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0378-1119(90)90085-6
- PMID
- 2170233
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 1990
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984359914502771
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