Inner hair cell Cre-expressing transgenic mouse

Mingyuan Li; Yong Tian; Bernd Fritzsch; Jiangang Gao; Xudong Wu; Jian Zuo

doi:10.1002/gene.20040

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Journal article

Inner hair cell Cre-expressing transgenic mouse

Mingyuan Li, Yong Tian, Bernd Fritzsch, Jiangang Gao, Xudong Wu and Jian Zuo

Genesis (New York, N.Y. : 2000), Vol.39(3), pp.173-177

07/2004

DOI: 10.1002/gene.20040

PMID: 15282743

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Abstract

Cochlear hair cells of the inner ear are mechanosensory transducers critical for sound reception in mammals. A mouse with a specific expression of Cre recombinase activity in hair cells is essential for hair cell-specific gene targeting. Here we report a transgenic mouse in which Cre activity is detected in inner hair cells, not in supporting cells, in the cochlea. The Cre activity was visualized with both X-gal staining and beta-galactosidase immunostaining in progeny of a cross between our Cre line and the reporter ROSA26R line. In inner hair cells, the Cre activity started at postnatal day 14 and was maintained throughout adulthood. Starting at postnatal day 50, a few outer hair cells in the outermost row of cochlear apical and middle turns displayed the Cre activity. In vestibular hair cells and spiral ganglia, the Cre activity was also detected. Cre activity was present in cells widely distributed throughout brain, testis, and retina, but was absent in many other tissues such as kidney, heart, liver, and intestine. This Cre mouse line can thus be used for conditional gene targeting in mature inner hair cells of the cochlea. genesis 39:173-177, 2004.

Immunohistochemistry

Galactosides

Blotting, Southern

Gene Targeting - methods

Animals

Hair Cells, Auditory, Inner - metabolism

beta-Galactosidase

Integrases - metabolism

Mice, Transgenic

Mice

Indoles

DNA Primers

Details

Title: Subtitle: Inner hair cell Cre-expressing transgenic mouse
Creators: Mingyuan Li - Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA
Yong Tian
Bernd Fritzsch
Jiangang Gao
Xudong Wu
Jian Zuo
Resource Type: Journal article
Publication Details: Genesis (New York, N.Y. : 2000), Vol.39(3), pp.173-177
DOI: 10.1002/gene.20040
PMID: 15282743
NLM abbreviation: Genesis
ISSN: 1526-954X
eISSN: 1526-968X
Publisher: United States
Grant note: MH61971 / NIMH NIH HHS DC04761 / NIDCD NIH HHS DC05168 / NIDCD NIH HHS DC00215 / NIDCD NIH HHS DC06471 / NIDCD NIH HHS EY12950 / NEI NIH HHS CA21765 / NCI NIH HHS
Language: English
Date published: 07/2004
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070621402771

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