Journal article
Inositol trisphosphate and ryanodine receptor signaling distinctly regulate neurite pathfinding in response to engineered micropatterned surfaces
PloS one, Vol.19(9), e0308389
09/05/2024
DOI: 10.1371/journal.pone.0308389
PMCID: PMC11376539
PMID: 39236043
Abstract
Micro and nanoscale patterning of surface features and biochemical cues have emerged as tools to precisely direct neurite growth into close proximity with next generation neural prosthesis electrodes. Biophysical cues can exert greater influence on neurite pathfinding compared to the more well studied biochemical cues; yet the signaling events underlying the ability of growth cones to respond to these microfeatures remain obscure. Intracellular Ca
2+
signaling plays a critical role in how a growth cone senses and grows in response to various cues (biophysical features, repulsive peptides, chemo-attractive gradients). Here, we investigate the role of inositol triphosphate (IP3) and ryanodine-sensitive receptor (RyR) signaling as sensory neurons (spiral ganglion neurons, SGNs, and dorsal root ganglion neurons, DRGNs) pathfind in response to micropatterned substrates of varied geometries. We find that IP3 and RyR signaling act in the growth cone as they navigate biophysical cues and enable proper guidance to biophysical, chemo-permissive, and chemo-repulsive micropatterns. In response to complex micropatterned geometries, RyR signaling appears to halt growth in response to both topographical features and chemo-repulsive cues. IP3 signaling appears to play a more complex role, as growth cones appear to sense the microfeatures in the presence of xestospongin C but are unable to coordinate turning in response to them. Overall, key Ca
2+
signaling elements, IP3 and RyR, are found to be essential for SGNs to pathfind in response to engineered biophysical and biochemical cues. These findings inform efforts to precisely guide neurite regeneration for improved neural prosthesis function, including cochlear implants.
Details
- Title: Subtitle
- Inositol trisphosphate and ryanodine receptor signaling distinctly regulate neurite pathfinding in response to engineered micropatterned surfaces
- Creators
- Joseph T. Vecchi - University of IowaMadeline Rhomberg - University of IowaC Allan Guymon - University of Iowa, Chemical and Biochemical EngineeringMarlan R. Hansen - University of Iowa
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.19(9), e0308389
- Publisher
- Public Library of Science
- DOI
- 10.1371/journal.pone.0308389
- PMID
- 39236043
- PMCID
- PMC11376539
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Grant note
- R01-DC012578 / ; F31-DC020371 / ; T32-GM007337 / ;
- Alternative title
- Inositol trisphosphate and ryanodine receptor signaling regulate distinctly regulate neurite pathfinding
- Language
- English
- Date published
- 09/05/2024
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
- Record Identifier
- 9984701859002771
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