Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes

S DeLisle; M J Welsh

doi:10.1016/S0021-9258(18)42391-5

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Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes

Journal article

Open access

Peer reviewed

Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes

S DeLisle and M J Welsh

The Journal of biological chemistry, Vol.267(12), pp.7963-7966

04/25/1992

DOI: 10.1016/S0021-9258(18)42391-5

PMID: 1569053

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Abstract

Stimuli which act through the second messenger inositol 1,4,5-trisphosphate (InsP3) often increase free intracellular Ca2+ concentration ([Ca2+]i) in a localized subcellular area. Actively propagated Ca2+ waves then extend this focal Ca2+ signal to other parts of the cell. To understand how cells may control the spatial distribution of Ca2+, we investigated the mechanism by which Ca2+ waves propagate through the cytoplasm of Xenopus oocytes. Heparin, which inhibits the binding of InsP3 to its receptor, prevented the migration of Ca2+ waves induced by a poorly metabolized InsP3 (InsP3S3). This result suggested that Ca2+ waves move through the cell via the serial release of Ca2+ from InsP3-sensitive stores. Interventions which caused a localized increase in [Ca2+]i without elevations of InsP3 did not trigger Ca2+ waves. In the presence of a Ins-P3S3, however, endogenously released or locally injected Ca2+ elicited Ca2+ waves. A cooperative interaction between Ca2+ and InsP3 may therefore be responsible for the propagation of Ca2+ waves.

Xanthenes

Xenopus

Animals

Cations, Divalent

Calcium - metabolism

Aniline Compounds

Inositol 1,4,5-Trisphosphate - metabolism

Fluorescent Dyes

Microscopy, Fluorescence

Ovum - metabolism

Details

Title: Subtitle: Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes
Creators: S DeLisle - Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242
M J Welsh
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.267(12), pp.7963-7966
DOI: 10.1016/S0021-9258(18)42391-5
PMID: 1569053
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Language: English
Date published: 04/25/1992
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
Record Identifier: 9984020865702771

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