Journal article
Insoluble interphotoreceptor matrix in human vitreous after rhematogenous retinal detachment
American journal of ophthalmology, Vol.123(3), p.386
03/01/1997
DOI: 10.1016/S0002-9394(14)70135-7
Abstract
Purpose
To determine whether insoluble interphotoreceptor matrix is present in the vitreous of human eyes after rhegmatogenous retinal detachment.
Methods
Vitreous aspirates were collected from 12 eyes of 12 patients during retinal reattachment surgery or membrane peeling for rhegmatogenous retinal detachment-related macular epiretinal membranes between 1 day and 5 months after patients' initial rhegmatogenous retinal detachment symptoms. The aspirates were pelleted by centrifugation, embedded in acrylamide, sectioned, and incubated with fluorescein isothiocyanate-conjugated peanut agglutinin and an interphotoreceptor matrix-specific polyclonal antibody, designated 1-89B.
Results
Before surgery, 11 of 12 eyes exhibited pigment in the anterior vitreous (Shafer's sign). Morphologic and histochemical characteristic profiles of insoluble interphotoreceptor matrix domains, bound by fluorescein isothiocyanate-conjugated peanut agglutinin or polyclonal antibody 1-89B, or both, were identified in vitreous aspirates from 11 of 12 patients.
Conclusions
The results of this study provide evidence that insoluble interphotoreceptor matrix constituents gain access to the vitreous after rhegmatogenous retinal detachment. Furthermore, insoluble interphotoreceptor matrix may remain in the vitreous for several months without being degraded.
Details
- Title: Subtitle
- Insoluble interphotoreceptor matrix in human vitreous after rhematogenous retinal detachment
- Creators
- Stephen R RussellGregory S Hageman
- Resource Type
- Journal article
- Publication Details
- American journal of ophthalmology, Vol.123(3), p.386
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0002-9394(14)70135-7
- ISSN
- 0002-9394
- eISSN
- 1879-1891
- Language
- English
- Date published
- 03/01/1997
- Description audience
- Professional
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984187149002771
Metrics
8 Record Views