Journal article
Insulin-like growth factor-I governs submucosal growth and thickness in the newborn mouse ileum
Pediatric research, Vol.55(3), pp.507-513
2004
DOI: 10.1203/01.PDR.0000110525.30786.50
PMID: 14681493
Abstract
Spontaneous intestinal perforations in extremely premature infants are associated with glucocorticoid-induced thinning of the ileal bowel wall. We have previously demonstrated that insulin-like growth factor-1 (IGF-1) is abundant within the submucosa of the newborn mouse ileum but is diminished by glucocorticoid exposure, concomitant with bowel wall thinning. These findings prompted us to hypothesize that IGF-I governs submucosal growth during neonatal gut development and that diminished IGF-I abundance results in submucosal thinning. Heterozygous IGF-I knockout, wild type and homozygous IGF-I over-expresser newborn mice were euthanized at 3 d of life. Additionally, wild type newborn mice received daily dexamethasone (DEX) (1microg/gm/d) or vehicle control on days of life 1 and 2 and were also euthanized at 3 d of life. Their ileums were harvested, fixed and the resulting sections were processed in parallel for IGF-I immunohistochemistry and morphometric measurements of submucosal thickness and bowel diameter. Immunolocalization of IGF-I in each genotype was also compared with that seen in DEX-treated and control mice euthanized at the same time of life. IGF-I heterozygous knockouts had diminished submucosal IGF-I immunolocalization (similar to that seen in DEX-treated newborn mice) whereas homozygous IGF-I over-expressers exceeded that seen within wild type mice. IGF-I genotype and submucosal abundance was positively correlated with ileal submucosal thickness. DEX treatment of newborn mice diminished IGF-I and caused significant thinning of the submucosa compared with controls. Submucosal growth and thickness in the neonatal mouse ileum is governed by IGF-I and is diminished by dexamethasone treatment.
Details
- Title: Subtitle
- Insulin-like growth factor-I governs submucosal growth and thickness in the newborn mouse ileum
- Creators
- Andrew C HERMAN - Division of Neonatology, Department of Pediatrics, University of Virginia Health System, Charlottesville, Virginia 22908, United StatesErica M CARLISLE - Division of Neonatology, Department of Pediatrics, University of Virginia Health System, Charlottesville, Virginia 22908, United StatesJessica B PAXTON - Division of Neonatology, Department of Pediatrics, University of Virginia Health System, Charlottesville, Virginia 22908, United StatesPhillip V GORDON - Division of Neonatology, Department of Pediatrics, University of Virginia Health System, Charlottesville, Virginia 22908, United States
- Resource Type
- Journal article
- Publication Details
- Pediatric research, Vol.55(3), pp.507-513
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- DOI
- 10.1203/01.PDR.0000110525.30786.50
- PMID
- 14681493
- ISSN
- 0031-3998
- eISSN
- 1530-0447
- Language
- English
- Date published
- 2004
- Academic Unit
- Stead Family Department of Pediatrics; Surgery
- Record Identifier
- 9984051559702771
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