Journal article
Insulin-resistance reduces botulinum neurotoxin-type A induced prostatic atrophy and apoptosis in rats
European journal of pharmacology, Vol.650(1), pp.356-363
01/10/2011
DOI: 10.1016/j.ejphar.2010.09.066
PMID: 20940009
Abstract
Botulinum neurotoxin-type A (BoNTA) is an emerging therapeutic option for the treatment of benign prostate hyperplasia Recent reports indicate increased incidence of benign prostate hyperplasia in the insulin-resistant individuals Insulin resistance is associated with the compensatory rise in the plasma insulin which is known to have growth-promoting effects The present study investigated the effect of insulin resistance on the effectiveness of BoNTA in inducing prostate atrophy in rats Sprague Dawley rats (200-220 g) maintained on normal-pellet or high-fat diet were injected in the ventral prostate with 200 ill of saline or the same volume containing 5U BoNTA at the end of 9 weeks and were sacrificed 3 weeks later Ventral prostate was carefully isolated, weighed fixed and stained to examine the cellular morphology cell death and proliferation High-fat diet produced insulin-resistance hyperinsulnemia and prostate enlargement in rats BoNTA caused prostate atrophy and apoptosis in both insulin resistant and insulin-sensitive rats However the effect of BoNTA was more prominent in insulin-sensitive rats (apoptosis 2 fold prostate atrophy-3 fold) as compared to the insulin-resistant rats Significant increase in the phosphorylation of ERK-1/2 and expression of the proliferating cell nuclear antigen was observed in the prostate of insulin resistant rats In the present investigation we report that diet-induced insulin-resistance activates mitogenic signaling of insulin increases cellular proliferation and reduces BoNTA-induced prostate atrophy and apoptosts in rats Results of the present study indicate that the insulin-resistance can affect the therapeutic outcome of BoNTA. (C) 2010 Elsevier B V All rights reserved
Details
- Title: Subtitle
- Insulin-resistance reduces botulinum neurotoxin-type A induced prostatic atrophy and apoptosis in rats
- Creators
- Ajit Vikram - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Mohali, Punjab-160 062, IndiaGopabandhu Jena - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Mohali, Punjab-160 062, IndiaPoduri Ramarao - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Mohali, Punjab-160 062, India
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.650(1), pp.356-363
- Publisher
- Elsevier
- DOI
- 10.1016/j.ejphar.2010.09.066
- PMID
- 20940009
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Number of pages
- 8
- Grant note
- National Institute of Pharmaceutical Education and Research (NIPER)
- Language
- English
- Date published
- 01/10/2011
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984360149502771
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