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Integrase-defective lentiviral vectors: progress and applications
Journal article   Peer reviewed

Integrase-defective lentiviral vectors: progress and applications

M. B BANASIK and P. B MCCRAY
Gene therapy, Vol.17(2), pp.150-157
2010
DOI: 10.1038/gt.2009.135
PMID: 19847206

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Abstract

Lentiviral vectors (LVs) offer the advantages of a large packaging capacity, broad cell tropism or specific cell-type targeting through pseudotyping, and long-term expression from integrated gene cassettes. However, transgene integration carries a risk of disrupting gene expression through insertional mutagenesis and may not be required for all applications. A non-integrating LV may be beneficial in cases in which transient gene expression is desired. Several recent publications outline the development and initial biological characterization of such vectors. Here, we discuss the potential applications and new directions for the development of integration-defective LVs.
Biotechnology Gene Therapy Fundamental and applied biological sciences. Psychology Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Transfusions. Complications. Transfusion reactions. Cell and gene therapy Applied cell therapy and gene therapy Biological and medical sciences Medical sciences

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