Journal article
Integrated methodological approach reveals microbial diversity and functions in aerobic groundwater microcosms adapted to vinyl chloride
FEMS microbiology ecology, Vol.94(9), fiy124
09/01/2018
DOI: 10.1093/femsec/fiy124
PMID: 29945195
Abstract
Vinyl chloride (VC), a known human carcinogen, is often formed in groundwater (GW) by incomplete reductive dechlorination of chlorinated ethenes. An integrated microbial ecology approach involving bacterial enrichments and isolations, carbon stable-isotope probing (SIP) and metagenome and genome sequencing was applied to ethene-fed GW microcosms that rapidly transitioned to aerobic growth on VC. Actinobacteria, Proteobacteria and Bacteroidetes dominated the microbial communities in ethene- and VC-grown cultures. SIP with 13C2-VC demonstrated that Nocardioides spp. significantly participated in carbon uptake from VC (52.1%-75.7% enriched in heavy fractions). Sediminibacterium, Pedobacter and Pseudomonas spp. also incorporated 13C from VC into genomic DNA. Ethene- and VC-assimilating Nocardioides sp. strain XL1 was isolated. Sequencing revealed a large (∼300 kbp) plasmid harboring genes encoding alkene monooxygenase and epoxyalkane: coenzyme M transferase, enzymes known to participate in aerobic VC and ethene biodegradation. The plasmid was 100% identical to pNOCA01 found in VC-assimilating Nocardioides sp. strain JS614. Metagenomic analysis of enrichment cultures indicated other bacteria implicated in carbon uptake from VC possessed the genetic potential to detoxify epoxides via epoxide hydrolase or glutathione S-transferase (Pseudomonas) and/or metabolize VC epoxide breakdown products and downstream VC metabolites. This study provides new functional insights into aerobic VC metabolism within a GW microbial community.
Details
- Title: Subtitle
- Integrated methodological approach reveals microbial diversity and functions in aerobic groundwater microcosms adapted to vinyl chloride
- Creators
- Xikun Liu - Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, IA, 52242, USAYang Wu - School of Environment and Energy, Peking University Shenzhen Graduate School, University Town, Nanshan District, Shenzhen 518055, ChinaFernanda P Wilson - Department of Civil and Environmental Engineering, Engineering Building, 428 S. Shaw Lane, Room 3546, East Lansing, MI 48824, USAKe Yu - School of Environment and Energy, Peking University Shenzhen Graduate School, University Town, Nanshan District, Shenzhen 518055, ChinaCarly Lintner - Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, IA, 52242, USAAlison M Cupples - Department of Civil and Environmental Engineering, Engineering Building, 428 S. Shaw Lane, Room 3546, East Lansing, MI 48824, USATimothy E Mattes - Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, IA, 52242, USA
- Resource Type
- Journal article
- Publication Details
- FEMS microbiology ecology, Vol.94(9), fiy124
- Publisher
- England
- DOI
- 10.1093/femsec/fiy124
- PMID
- 29945195
- ISSN
- 0168-6496
- eISSN
- 1574-6941
- Language
- English
- Date published
- 09/01/2018
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9984001081902771
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