Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth

Sanjeeva Metikala; Michael Warkala; Satish Casie Chetty; Brendan Chestnut; Diandra Rufin Florat; Elizabeth Plender; Olivia Nester; Andrew L Koenig; Sophie Astrof; Saulius Sumanas

doi:10.1016/j.devcel.2022.02.015

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Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth

Journal article

Peer reviewed

Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth

Sanjeeva Metikala, Michael Warkala, Satish Casie Chetty, Brendan Chestnut, Diandra Rufin Florat, Elizabeth Plender, Olivia Nester, Andrew L Koenig, Sophie Astrof and Saulius Sumanas

Developmental cell, Vol.57(6), pp.767-782.e6

03/28/2022

DOI: 10.1016/j.devcel.2022.02.015

PMCID: PMC9365108

PMID: 35276066

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Abstract

During embryogenesis, the initial vascular network forms by the process of vasculogenesis, or the specification of vascular progenitors de novo. In contrast, the majority of later-forming vessels arise by angiogenesis from the already established vasculature. Here, we show that new vascular progenitors in zebrafish embryos emerge from a distinct site along the yolk extension, or secondary vascular field (SVF), incorporate into the posterior cardinal vein, and contribute to subintestinal vasculature even after blood circulation has been initiated. We further demonstrate that SVF cells participate in vascular recovery after chemical ablation of vascular endothelial cells. Inducible inhibition of the function of vascular progenitor marker etv2/etsrp prevented SVF cell differentiation and resulted in the defective formation of subintestinal vasculature. Similar late-forming etv2+ progenitors were also observed in mouse embryos, suggesting that SVF cells are evolutionarily conserved. Our results characterize a distinct mechanism by which new vascular progenitors incorporate into established vasculature.

Animals

Blood Vessels - metabolism

Embryo, Nonmammalian - metabolism

Endothelial Cells - metabolism

Gene Expression Regulation, Developmental

Mice

Neovascularization, Physiologic

Zebrafish - metabolism

Zebrafish Proteins - metabolism

Details

Title: Subtitle: Integration of vascular progenitors into functional blood vessels represents a distinct mechanism of vascular growth
Creators: Sanjeeva Metikala
Michael Warkala - Rutgers Health
Satish Casie Chetty
Brendan Chestnut - Cincinnati Children's Hospital Medical Center
Diandra Rufin Florat - University of South Florida
Elizabeth Plender - Cincinnati Children's Hospital Medical Center
Olivia Nester - Cincinnati Children's Hospital Medical Center
Andrew L Koenig
Sophie Astrof - Rutgers Health
Saulius Sumanas
Resource Type: Journal article
Publication Details: Developmental cell, Vol.57(6), pp.767-782.e6
DOI: 10.1016/j.devcel.2022.02.015
PMID: 35276066
PMCID: PMC9365108
NLM abbreviation: Dev Cell
ISSN: 1534-5807
eISSN: 1878-1551
Grant note: R01 HL103920 / NHLBI NIH HHS R01 HL134935 / NHLBI NIH HHS R21 AI128445 / NIAID NIH HHS R01 HL153005 / NHLBI NIH HHS F31 HL135986 / NHLBI NIH HHS R01 HL158049 / NHLBI NIH HHS
Language: English
Date published: 03/28/2022
Academic Unit: Stead Family Department of Pediatrics
Record Identifier: 9985161355502771

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