Integration site choice of a feline immunodeficiency virus vector

Yubin Kang; Christopher J Moressi; Todd E Scheetz; Litao Xie; Diane Thi Tran; Thomas L Casavant; Prashanth Ak; Craig J Benham; Beverly L Davidson; Paul B McCray Jr

doi:10.1128/JVI.00719-06

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Integration site choice of a feline immunodeficiency virus vector

Journal article

Open access

Peer reviewed

Integration site choice of a feline immunodeficiency virus vector

Yubin Kang, Christopher J Moressi, Todd E Scheetz, Litao Xie, Diane Thi Tran, Thomas L Casavant, Prashanth Ak, Craig J Benham, Beverly L Davidson and Paul B McCray Jr

Journal of virology, Vol.80(17), pp.8820-8823

09/2006

DOI: 10.1128/JVI.00719-06

PMCID: PMC1563849

PMID: 16912328

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https://doi.org/10.1128/JVI.00719-06View

Published (Version of record) Open Access

Abstract

We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a "bendable" structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

Cats

Oligonucleotide Array Sequence Analysis

Humans

Chromosome Mapping

Gene Expression Profiling

Virus Integration

Proteins - genetics

Animals

Proteins - metabolism

Immunodeficiency Virus, Feline - genetics

Cell Line, Tumor

Immunodeficiency Virus, Feline - pathogenicity

Genetic Vectors

Details

Title: Subtitle: Integration site choice of a feline immunodeficiency virus vector
Creators: Yubin Kang - Program in Gene Therapy, Department of Pediatrics, University of Iowa, Iowa City, 52242, 52242, USA
Christopher J Moressi
Todd E Scheetz
Litao Xie
Diane Thi Tran - University of Iowa
Thomas L Casavant
Prashanth Ak
Craig J Benham
Beverly L Davidson
Paul B McCray Jr
Resource Type: Journal article
Publication Details: Journal of virology, Vol.80(17), pp.8820-8823
DOI: 10.1128/JVI.00719-06
PMID: 16912328
PMCID: PMC1563849
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: HL-51670 / NHLBI NIH HHS HL-75363 / NHLBI NIH HHS R01 HL079023 / NHLBI NIH HHS R01 HL075363 / NHLBI NIH HHS R01 GM068903 / NIGMS NIH HHS P01 HL051670 / NHLBI NIH HHS HL-79023 / NHLBI NIH HHS
Language: English
Date published: 09/2006
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983980091202771

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