Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry

Ernst Hafen; Lee Hood; Michael G Katze; Kathleen A Kennedy; Floyd Kregenow; Hookeun Lee; Biaoyang Lin; Dan Martin; Jeffrey A Ranish; Frank Desiere; David J Rawlings; Eric W Deutsch; Lawrence E Samelson; Alexey I Nesvizhskii; Yuzuru Shiio; Julian D Watts; Parag Mallick; Nichole L King; Michael E Wright; Jimmy K Eng; Bernd Wollscheid; Wei Yan; Alan Aderem; Rose Boyle; Lihong Yang; Erich Brunner; Eugene C Yi; Samuel Donohoe; Hui Zhang; Nelson Fausto; Ruedi Aebersold

doi:10.1186/gb-2004-6-1-r9

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Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry

Journal article

Open access

Peer reviewed

Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry

Ernst Hafen, Lee Hood, Michael G Katze, Kathleen A Kennedy, Floyd Kregenow, Hookeun Lee, Biaoyang Lin, Dan Martin, Jeffrey A Ranish, Frank Desiere, …

Genome biology, Vol.6(1), R9

12/10/2004

DOI: 10.1186/gb-2004-6-1-r9

PMCID: PMC549070

PMID: 15642101

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Abstract

A crucial aim upon the completion of the human genome is the verification and functional annotation of all predicted genes and their protein products. Here we describe the mapping of peptides derived from accurate interpretations of protein tandem mass spectrometry (MS) data to eukaryotic genomes and the generation of an expandable resource for integration of data from many diverse proteomics experiments. Furthermore, we demonstrate that peptide identifications obtained from high-throughput proteomics can be integrated on a large scale with the human genome. This resource could serve as an expandable repository for MS-derived proteome information.

Computational Biology

Software

Amino Acid Sequence

Humans

Peptides - genetics

Eukaryotic Cells - metabolism

Proteome

Drosophila melanogaster - genetics

Animals

Drosophila melanogaster - chemistry

Databases, Protein

Proteomics - methods

Genome, Human

Mass Spectrometry - methods

Peptides - analysis

Details

Title: Subtitle: Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry
Creators: Ernst Hafen
Lee Hood
Michael G Katze
Kathleen A Kennedy
Floyd Kregenow
Hookeun Lee
Biaoyang Lin
Dan Martin
Jeffrey A Ranish
Frank Desiere
David J Rawlings
Eric W Deutsch
Lawrence E Samelson
Alexey I Nesvizhskii
Yuzuru Shiio
Julian D Watts
Parag Mallick
Nichole L King
Michael E Wright - University of Iowa, Molecular Physiology and Biophysics
Jimmy K Eng
Bernd Wollscheid
Wei Yan
Alan Aderem
Rose Boyle
Lihong Yang
Erich Brunner
Eugene C Yi
Samuel Donohoe
Hui Zhang
Nelson Fausto
Ruedi Aebersold
Resource Type: Journal article
Publication Details: Genome biology, Vol.6(1), R9
Publisher: England
DOI: 10.1186/gb-2004-6-1-r9
PMID: 15642101
PMCID: PMC549070
ISSN: 1474-760X
eISSN: 1474-760X
Grant note: R01 AI051344 / NIAID NIH HHS N01HV28179 / NHLBI NIH HHS N01-HV-28179 / NHLBI NIH HHS P30DA015625 / NIDA NIH HHS P30 DA015625 / NIDA NIH HHS R01-AI-51344-01 / NIAID NIH HHS
Language: English
Date published: 12/10/2004
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9983759098102771

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