Journal article
Integrative Bioinformatics Analysis of Genomic and Proteomic Approaches to Understand the Transcriptional Regulatory Program in Coronary Artery Disease Pathways
PloS one, Vol.8(2), e57193
02/28/2013
DOI: 10.1371/journal.pone.0057193
PMCID: PMC3585295
PMID: 23468932
Abstract
Patients with cardiovascular disease show a panel of differentially regulated serum biomarkers indicative of modulation of several pathways from disease onset to progression. Few of these biomarkers have been proposed for multimarker risk prediction methods. However, the underlying mechanism of the expression changes and modulation of the pathways is not yet addressed in entirety. Our present work focuses on understanding the regulatory mechanisms at transcriptional level by identifying the core and specific transcription factors that regulate the coronary artery disease associated pathways. Using the principles of systems biology we integrated the genomics and proteomics data with computational tools. We selected biomarkers from 7 different pathways based on their association with the disease and assayed 24 biomarkers along with gene expression studies and built network modules which are highly regulated by 5 core regulators PPARG, EGR1, ETV1, KLF7 and ESRRA. These network modules in turn comprise of biomarkers from different pathways showing that the core regulatory transcription factors may work together in differential regulation of several pathways potentially leading to the disease. This kind of analysis can enhance the elucidation of mechanisms in the disease and give better strategies of developing multimarker module based risk predictions.
Details
- Title: Subtitle
- Integrative Bioinformatics Analysis of Genomic and Proteomic Approaches to Understand the Transcriptional Regulatory Program in Coronary Artery Disease Pathways
- Creators
- Rajani Kanth Vangala - Thrombosis Research InstituteVandana Ravindran - Narayana HealthMadan Ghatge - Narayana Hrudayalaya Hosp, Thrombosis Res Inst, Tata Prote & Coagulat Dept, Bangalore, Karnataka, IndiaJayashree Shanker - Thrombosis Research InstitutePrathima Arvind - Thrombosis Research InstituteHima Bindu - Narayana HealthMeghala Shekar - Narayana HealthVeena S. Rao - Narayana Health
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.8(2), e57193
- DOI
- 10.1371/journal.pone.0057193
- PMID
- 23468932
- PMCID
- PMC3585295
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 9
- Grant note
- BT/01/COE/08/07 / Department of Biotechnology, Government of India; Department of Biotechnology (DBT) India
- Language
- English
- Date published
- 02/28/2013
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984958618202771
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