Journal article
Integrin alpha v beta 5 selectively promotes adenovirus mediated cell membrane permeabilization
The Journal of cell biology, Vol.127(1), pp.257-264
10/01/1994
DOI: 10.1083/jcb.127.1.257
PMCID: PMC2120185
PMID: 7523420
Abstract
Human adenovirus type 2 (Ad2) enters host cells by receptor-mediated endocytosis, an event mediated by the virus penton base binding to cell surface integrins alpha v beta 3 and alpha v beta 5. While both alpha v integrins promote virus internalization, alpha v beta 5 is involved in the subsequent event of membrane permeabilization. Cells transfected with the beta 5 or beta 3 subunit, expressing either alpha v beta 5 and alpha v beta 3, respectively, were capable of supporting Ad2 infection to varying degrees. In this case, cells expressing alpha v beta 5 were significantly more susceptible to Ad2-induced membrane permeabilization, as well as to Ad2 infection, than cells expressing alpha v beta 3. Adenovirus-mediated gene delivery was also more efficient in cells expressing alpha v beta 5. These results suggest that the interaction of alpha v beta 5 with Ad2 penton base facilitates the subsequent step of virus penetration into the cell. These studies provide evidence for the involvement of a cellular receptor in virus-mediated membrane permeabilization and suggest a novel biological role for integrin alpha v beta 5 in the infectious pathway of a human adenovirus.
Details
- Title: Subtitle
- Integrin alpha v beta 5 selectively promotes adenovirus mediated cell membrane permeabilization
- Creators
- T J Wickham - Department of Immunology, Scripps Research Institute, La Jolla, California 92037E J Filardo - Department of Immunology, Scripps Research Institute, La Jolla, California 92037D A Cheresh - Department of Immunology, Scripps Research Institute, La Jolla, California 92037G R Nemerow - Department of Immunology, Scripps Research Institute, La Jolla, California 92037
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.127(1), pp.257-264
- DOI
- 10.1083/jcb.127.1.257
- PMID
- 7523420
- PMCID
- PMC2120185
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 10/01/1994
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
- Record Identifier
- 9984051887002771
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