Journal article
Integrin alpha(v)beta(3) as a PET Imaging Biomarker for Osteoclast Number in Mouse Models of Negative and Positive Osteoclast Regulation
Molecular imaging and biology, Vol.14(4), pp.500-508
08/01/2012
DOI: 10.1007/s11307-011-0512-4
PMCID: PMC4277818
PMID: 21853370
Abstract
The goal of this study was to determine the specificity of Cu-64-CB-TE2A-c(RGDyK) (Cu-64-RGD) for osteoclast-related diseases, such as Paget's disease or rheumatoid arthritis.
C57BL/6 mice were treated systemically with osteoprotegerin (OPG) for 15 days or RANKL for 11 days to suppress and stimulate osteoclastogenesis, respectively. The mice were then imaged by positron emission tomography/computed tomography using Cu-64-RGD, followed by determination of serum TRAP5b and bone histology. Standard uptake values were determined to quantify Cu-64-RGD in bones and other tissues.
Mice treated with OPG showed decreased bone uptake of Cu-64-RGD at 1, 2, and 24 h post-injection of the tracer (p < 0.01 for all time points) compared to vehicle controls, which correlated with a post-treatment decrease in serum TRAP5b. In contrast, mice treated with RANKL showed significantly increased bone uptake at 2 h post-injection of Cu-64-RGD (p < 0.05) compared to the vehicle control group, corresponding to increased serum TRAP5b and OC numbers as determined by bone histology.
These data demonstrate that Cu-64-RGD localizes to areas in bone with increased osteoclast numbers and support the use of Cu-64-RGD as an imaging biomarker for osteoclast number that could be used to monitor osteoclast-related pathologies and their treatments.
Details
- Title: Subtitle
- Integrin alpha(v)beta(3) as a PET Imaging Biomarker for Osteoclast Number in Mouse Models of Negative and Positive Osteoclast Regulation
- Creators
- Alexander Zheleznyak - MallinckrodtThaddeus J. Wadas - Wake Forest UniversityChristopher D. Sherman - MallinckrodtJessica M. Wilson - MallinckrodtPaul J. Kostenuik - AmgenKatherine N. Weilbaecher - Washington University in St. LouisCarolyn J. Anderson - University of Pittsburgh
- Resource Type
- Journal article
- Publication Details
- Molecular imaging and biology, Vol.14(4), pp.500-508
- Publisher
- Springer Nature
- DOI
- 10.1007/s11307-011-0512-4
- PMID
- 21853370
- PMCID
- PMC4277818
- ISSN
- 1536-1632
- eISSN
- 1860-2002
- Number of pages
- 9
- Grant note
- Amgen, Inc.; Amgen NRSA F32 CA115148; R01 CA097250; P01 CA100730 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA F32CA115148 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 08/01/2012
- Academic Unit
- Radiology; Radiation Oncology
- Record Identifier
- 9984312981402771
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