Integrin-associated tyrosine kinase FAK affects Cbfa1 expression

Galen B Schneider; Rebecca Zaharias; Denise Seabold; Clark Stanford

doi:10.1002/jor.21382

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Integrin-associated tyrosine kinase FAK affects Cbfa1 expression

Journal article

Peer reviewed

Integrin-associated tyrosine kinase FAK affects Cbfa1 expression

Galen B Schneider, Rebecca Zaharias, Denise Seabold and Clark Stanford

Journal of orthopaedic research, Vol.29(9), pp.1443-1447

09/2011

DOI: 10.1002/jor.21382

PMID: 21412826

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Abstract

Following cell adhesion, focal adhesion kinase (FAK) autophosphorylates on tyrosine and regulates intracellular signaling cascades that regulate cell growth and differentiation. The hypothesis of this study was FAK mediates osteoblast differentiation dependent Cbfa1 expression. Slowly mineralizing UI and rapidly mineralizing UMR-106-01 BSP osteoblasts formed focal adhesions; however, the level of FAK in UI focal adhesions was less than that seen in BSP cells. UI cultures had less FAK expression (p < 0.05) along with elevated levels of FAK phosphotyrosine in comparison to rapidly mineralizing BSP cultures. Mineralization decreased in a dose-dependent manner in response to Herbimycin A, a tyrosine kinase inhibitor. Overexpression of FAK in UI cells led to a fourfold increase in Cbfa1 gene expression (p < 0.02), and an increase in Cbfa1 protein expression. These results suggest that the integrin-associated tyrosine kinase FAK contributes to the regulation of the osteoblast differentiation in part through the regulation of Cbfa1 expression.

Phosphorylation

Calcification, Physiologic - drug effects

Osteoblasts - enzymology

Focal Adhesion Protein-Tyrosine Kinases - metabolism

Osteoblasts - drug effects

Rifabutin - analogs & derivatives

Cells, Cultured

Enzyme Inhibitors - pharmacology

Calcification, Physiologic - physiology

Rats

Lactams, Macrocyclic - pharmacology

RNA, Messenger - metabolism

Core Binding Factor Alpha 1 Subunit - metabolism

Integrins - metabolism

Benzoquinones - pharmacology

Gene Expression Regulation - drug effects

Cell Differentiation - genetics

Animals

Cell Differentiation - drug effects

Osteoblasts - cytology

Core Binding Factor Alpha 1 Subunit - genetics

Protein-Tyrosine Kinases - antagonists & inhibitors

Details

Title: Subtitle: Integrin-associated tyrosine kinase FAK affects Cbfa1 expression
Creators: Galen B Schneider - Department of Prosthodontics, University of Iowa College of Dentistry, The University of Iowa, Iowa City, Iowa 52242-1001, USA. galen-schneider@uiowa.edu
Rebecca Zaharias
Denise Seabold
Clark Stanford
Resource Type: Journal article
Publication Details: Journal of orthopaedic research, Vol.29(9), pp.1443-1447
DOI: 10.1002/jor.21382
PMID: 21412826
NLM abbreviation: J Orthop Res
ISSN: 0736-0266
eISSN: 1554-527X
Publisher: United States
Grant note: R03DE014269 / NIDCR NIH HHS P60DE13076 / NIDCR NIH HHS
Language: English
Date published: 09/2011
Academic Unit: Anatomy and Cell Biology; Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center
Record Identifier: 9984065986902771

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