Integrin-based adhesions promote cell-cell junction and cytoskeletal remodelling to drive embryonic wound healing

Michelle Ly; Clara Schimmer; Raymond Hawkins; Katheryn E Rothenberg; Rodrigo Fernandez-Gonzalez

doi:10.1242/jcs.261138

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Integrin-based adhesions promote cell-cell junction and cytoskeletal remodelling to drive embryonic wound healing

Journal article

Peer reviewed

Integrin-based adhesions promote cell-cell junction and cytoskeletal remodelling to drive embryonic wound healing

Michelle Ly, Clara Schimmer, Raymond Hawkins, Katheryn E Rothenberg and Rodrigo Fernandez-Gonzalez

Journal of cell science, Vol.137(5), 261138

03/01/2024

DOI: 10.1242/jcs.261138

PMID: 37970744

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Abstract

Embryos repair wounds rapidly, with no inflammation or scarring. Embryonic wound healing is driven by the collective movement of the cells around the lesion. The cells adjacent to the wound polarize the cytoskeletal protein actin and the molecular motor non-muscle myosin II, which accumulate at the wound edge forming a supracellular cable around the wound. Adherens junction proteins, including E-cadherin, are internalized from the wound edge and localize to former tricellular junctions at the wound margin, in a process necessary for cytoskeletal polarity. We found that the cells adjacent to wounds in the Drosophila embryonic epidermis polarized Talin, a core component of cell-extracellular matrix (ECM) adhesions, which preferentially accumulated at the wound edge. Integrin knockdown and inhibition of integrin binding delayed wound closure and reduced actin polarization and dynamics around the wound. Additionally, disrupting integrins caused a defect in E-cadherin reinforcement at tricellular junctions along the wound edge, suggesting crosstalk between integrin-based and cadherin-based adhesions. Our results show that cell-ECM adhesion contributes to embryonic wound repair and reveal an interplay between cell-cell and cell-ECM adhesion in the collective cell movements that drive rapid wound healing.

Actins - metabolism

Animals

Cadherins - metabolism

Cell Adhesion

Cell Movement - physiology

Drosophila - metabolism

Integrins - metabolism

Intercellular Junctions - metabolism

Wound Healing - physiology

Details

Title: Subtitle: Integrin-based adhesions promote cell-cell junction and cytoskeletal remodelling to drive embryonic wound healing
Creators: Michelle Ly - Ted Rogers Centre for Heart Research
Clara Schimmer - University of Toronto
Raymond Hawkins - University of Toronto
Katheryn E Rothenberg - University of Toronto
Rodrigo Fernandez-Gonzalez - Hospital for Sick Children
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.137(5), 261138
DOI: 10.1242/jcs.261138
PMID: 37970744
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Grant note: 156279 / CIHR 156279, 186188 / CIHR
Language: English
Date published: 03/01/2024
Academic Unit: Biology
Record Identifier: 9984696719102771

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