Integrins contribute to initial morphological development and process outgrowth in rat adult hippocampal progenitor cells

Matthew M Harper; Eun-Ah Ye; Christopher C Blong; Mark L Jacobson; Donald S Sakaguchi

doi:10.1007/s12031-009-9211-x

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Integrins contribute to initial morphological development and process outgrowth in rat adult hippocampal progenitor cells

Journal article

Peer reviewed

Integrins contribute to initial morphological development and process outgrowth in rat adult hippocampal progenitor cells

Matthew M Harper, Eun-Ah Ye, Christopher C Blong, Mark L Jacobson and Donald S Sakaguchi

Journal of molecular neuroscience, Vol.40(3), pp.269-283

03/2010

DOI: 10.1007/s12031-009-9211-x

PMID: 19499350

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Abstract

Adult rat hippocampal progenitor cells (AHPCs) are self-renewing, multipotent neural progenitor cells (NPCs) that can differentiate into neurons, oligodendrocytes, and astrocytes. AHPCs contact a variety of molecular cues within their surrounding microenvironment via integrins. We hypothesize that integrin receptors are important for NPCs. In this study, we have examined the distribution of integrins in neuronal-like, oligodendrocyte-like, and astrocyte-like AHPCs when grown on substrates that support integrin-mediated adhesion (laminin, fibronectin), and those that do not (poly-L: -ornithine, PLO) using immunocytochemistry as well as characterized the phenotypic differentiation of AHPCs plated on laminin and fibronectin. Focal adhesions were prominent in AHPCs plated on purified substrates, but were also found in AHPCs plated on PLO. The focal adhesions observed in AHPCs plated on PLO substrates may be formed by self-adhesion to the endogenously produced laminin or fibronectin. We have demonstrated that integrins contribute to the initial morphological differentiation of AHPCs, as inhibition of fibronectin binding with the competitive inhibitor echistatin significantly decreased the number of processes and microspikes present in treated cells, and also decreased overall cell area. Finally, we have characterized the genetic profile of a subset of integrins and integrin-related genes in the AHPCs using reverse transcriptase polymerase chain reaction. These results demonstrate an important role of integrins, in vitro, for the initial morphological differentiation of AHPCs. Supplemental material available in online version.

Phenotype

Cell Surface Extensions - ultrastructure

Cell Surface Extensions - metabolism

Rats, Inbred F344

Cells, Cultured

Extracellular Matrix - metabolism

Integrins - genetics

Rats

Hippocampus - cytology

Integrins - metabolism

Extracellular Matrix - chemistry

Fibronectins - metabolism

Protein Subunits - metabolism

Animals

Peptides - metabolism

Multipotent Stem Cells - cytology

Cell Shape

Cytoskeleton - metabolism

Laminin - metabolism

Platelet Aggregation Inhibitors - metabolism

Multipotent Stem Cells - physiology

Cell Differentiation - physiology

Morphogenesis - physiology

Protein Subunits - genetics

Details

Title: Subtitle: Integrins contribute to initial morphological development and process outgrowth in rat adult hippocampal progenitor cells
Creators: Matthew M Harper - Iowa State University, Ames, IA 50011, USA
Eun-Ah Ye
Christopher C Blong
Mark L Jacobson
Donald S Sakaguchi
Resource Type: Journal article
Publication Details: Journal of molecular neuroscience, Vol.40(3), pp.269-283
Publisher: United States
DOI: 10.1007/s12031-009-9211-x
PMID: 19499350
ISSN: 0895-8696
eISSN: 1559-1166
Language: English
Date published: 03/2010
Academic Unit: Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983979921102771

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26 Times Cited - Web of Science