Journal article
Integrity of the Mod(mdg4)-67.2 BTB Domain Is Critical to Insulator Function in Drosophila melanogaster
Molecular and cellular biology, Vol.27(3), pp.963-974
02/01/2007
DOI: 10.1128/MCB.00795-06
PMCID: PMC1800699
PMID: 17101769
Abstract
ABSTRACT The Drosophila gypsy insulator contains binding sites for the Suppressor of Hairy-wing [Su(Hw)] protein. Enhancer and silencer blocking require Su(Hw) recruitment of Mod(mdg4)-67.2, a BTB/POZ domain protein that interacts with Su(Hw) through a carboxyl-terminal acidic domain. Here we conducted mutational analyses of the Mod(mdg4)-67.2 BTB domain. We demonstrate that this domain is essential for insulator function, in part through direction of protein dimerization. Our studies revealed the presence of a second domain (DD) that contributes to Mod(mdg4)-67.2 dimerization when the function of the BTB domain is compromised. Additionally, we demonstrate that mutations in amino acids of the charged pocket in the BTB domain that retain dimerization of the mutated protein cause a loss of insulator function. In these cases, the mutant proteins failed to localize to chromosomes, suggesting a role for the BTB domain in chromosome association. Interestingly, replacement of the Mod(mdg4)-67.2 BTB domain with the GAF BTB domain produced a nonfunctional protein. Taken together, these data suggest that the Mod(mdg4)-67.2 BTB domain confers novel activities to gypsy insulator function.
Details
- Title: Subtitle
- Integrity of the Mod(mdg4)-67.2 BTB Domain Is Critical to Insulator Function in Drosophila melanogaster
- Creators
- Anton Golovnin - Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia, University of Oslo, Centre for Medical Studies in Russia, Moscow 119334, RussiaAlexander Mazur - Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, RussiaMarina Kopantseva - Institute of Molecular Biology, Moscow 119334, RussiaMaria Kurshakova - Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, RussiaPavel V Gulak - Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, RussiaBrian Gilmore - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242William G. F Whitfield - Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, Scotland, United KingdomPamela Geyer - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Vincenzo Pirrotta - Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854Pavel Georgiev - Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.27(3), pp.963-974
- DOI
- 10.1128/MCB.00795-06
- PMID
- 17101769
- PMCID
- PMC1800699
- NLM abbreviation
- Mol Cell Biol
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 02/01/2007
- Academic Unit
- Obstetrics and Gynecology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025398802771
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