Interaction between interleukin-1 receptor 2 and Toll-like receptor 4, and cervical cytokines

Kelli K Ryckman; Scott M Williams; Marijane A Krohn; Hyagriv N Simhan

doi:10.1016/j.jri.2011.03.007

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Interaction between interleukin-1 receptor 2 and Toll-like receptor 4, and cervical cytokines

Journal article

Open access

Peer reviewed

Interaction between interleukin-1 receptor 2 and Toll-like receptor 4, and cervical cytokines

Kelli K Ryckman, Scott M Williams, Marijane A Krohn and Hyagriv N Simhan

Journal of reproductive immunology, Vol.90(2), pp.220-226

2011

DOI: 10.1016/j.jri.2011.03.007

PMCID: PMC3147278

PMID: 21704385

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Abstract

The objective was to assess the impact of genetic variation on cervical cytokine concentrations of interleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor alpha (TNF-α), and first, to determine if these variants interact with polymorphisms in Toll-like receptor 4 (TLR4) that were previously shown to associate with pro-inflammatory cervical cytokine concentrations, and second, to determine if findings are affected by bacterial vaginosis (BV). We examined 183 single nucleotide polymorphisms (SNPs) in 13 cytokine genes and receptors for associations with cervical cytokine levels in 188 African American and European American women. We tested for associations of gene–gene interactions between SNPs in TLR4 and cytokine gene and receptor polymorphisms with cervical pro-inflammatory cytokines. None of the single locus associations were significant after correction for multiple testing in either European Americans or African Americans. However, there were significant gene–gene interactions between IL-1R2 rs485127 and two SNPs in TLR4 (rs1554973 and rs7856729) with IL-1β after correction for multiple testing. Our study demonstrates that interactions between TLR4 and IL-1R2 are associated with cervical pro-inflammatory cytokine concentrations. These results provide important insights into the possible regulatory mechanisms of the inflammatory response in the presence and absence of microbial disorders such as BV. Additionally, the observed differences in allele frequencies between African Americans and those of European descent may partially explain population disparity in pregnancy-related phenotypes that are cytokine concentration-dependent.

Bacterial vaginosis

Cytokine concentrations

Ethnic disparity

Details

Title: Subtitle: Interaction between interleukin-1 receptor 2 and Toll-like receptor 4, and cervical cytokines
Creators: Kelli K Ryckman - Vanderbilt University School of Medicine, Department of Molecular Physiology and Biophysics, Center for Human Genetics, Nashville, TN, USA
Scott M Williams - Vanderbilt University School of Medicine, Department of Molecular Physiology and Biophysics, Center for Human Genetics, Nashville, TN, USA
Marijane A Krohn - University of Pittsburgh School of Medicine Magee-Women's Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences Division of Maternal-Fetal Medicine, Pittsburgh, PA, USA
Hyagriv N Simhan - University of Iowa Department of Pediatrics, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Journal of reproductive immunology, Vol.90(2), pp.220-226
DOI: 10.1016/j.jri.2011.03.007
PMID: 21704385
PMCID: PMC3147278
NLM abbreviation: J Reprod Immunol
ISSN: 0165-0378
eISSN: 1872-7603
Publisher: Elsevier Ireland Ltd
Language: English
Date published: 2011
Academic Unit: Stead Family Department of Pediatrics; Epidemiology
Record Identifier: 9984214695202771

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