Journal article
Interaction of human platelets and leukocytes in modulation of vascular tone
American journal of physiology. Heart and circulatory physiology, Vol.266(5), pp.H1706-H1714
05/01/1994
DOI: 10.1152/ajpheart.1994.266.5.H1706
PMID: 8203570
Abstract
We tested the hypothesis that the vasodilator response to human platelets is modulated by polymorphonuclear leukocytes (PMNs). Responses to platelets activated with thrombin, as well as PMNs activated with N-formylmethionyl-leucyl-phenylalanine (FMLP), were examined in perfused rabbit carotid arteries in vitro. Activation of platelets produced marked dilatation, and activation of PMNs produced modest constriction in arteries preconstricted with phenylephrine. Vasodilator responses to platelets were greatly impaired during infusion of activated PMNs. Pretreatment of PMNs with superoxide dismutase (SOD) partially restored dilator responses to platelets. Because SOD only partially restored vasodilator responses to platelets, we tested the possibility that adenosine-diphosphatase (ADPase) activity of PMNs may degrade ADP released by platelets and thus reduce vasodilator responses. After incubation with PMNs, dilator responses to ADP, but not acetylcholine, were significantly impaired. These findings indicate that vasodilatation produced by activated human platelets is profoundly impaired by activated leukocytes. We conclude that two mechanisms may account for this effect: 1) endothelium-derived relaxing factor, released in response to platelet-derived ADP, is inactivated by superoxide anion generated by activated PMNs and 2) ADP is degraded by ADPase activity of PMNs. We speculate that platelet-leukocyte interaction may have important effects on vasomotor tone.
Details
- Title: Subtitle
- Interaction of human platelets and leukocytes in modulation of vascular tone
- Creators
- Sanjay Kaul - Department of Internal Medicine, University of Iowa College ofMedicine, Iowa City 52242Barry J Waack - Department of Internal Medicine, University of Iowa College ofMedicine, Iowa City 52242Richard C Padgett - Department of Internal Medicine, University of Iowa College ofMedicine, Iowa City 52242Robert M Brooks - Department of Internal Medicine, University of Iowa College ofMedicine, Iowa City 52242Donald D Heistad - Department of Internal Medicine, University of Iowa College ofMedicine, Iowa City 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.266(5), pp.H1706-H1714
- DOI
- 10.1152/ajpheart.1994.266.5.H1706
- PMID
- 8203570
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 05/01/1994
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040335702771
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