Journal article
Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA
Nucleic acids research, Vol.26(23), pp.5388-5393
12/01/1998
DOI: 10.1093/nar/26.23.5388
PMCID: PMC148005
PMID: 9826763
Abstract
Replication protein A (RPA), a heterotrimeric single-stranded DNA binding protein, is required for recombination, and stimulates homologous pairing and DNA strand exchange promoted in vitro by human recombination protein HsRad51. Co-immunoprecipitation revealed that purified RPA interacts physically with HsRad51, as well as with HsDmc1, the homolog that is expressed specifically in meiosis. The interaction with HsRad51 was mediated by the 70 kDa subunit of RPA, and according to experiments with deletion mutants, this interaction required amino acid residues 169-326. In exponentially growing mammalian cells, 22% of nuclei showed foci of RPA protein and 1-2% showed foci of Rad51. After gamma-irradiation, the percentage of cells with RPA foci increased to approximately 50%, and those with Rad51 foci to 30%. All of the cells with foci of Rad51 had foci of RPA, and in those cells the two proteins co-localized in a high fraction of foci. The interactions of human RPA with Rad51, replication proteins and DNA are suited to the linking of recombination to replication.
Details
- Title: Subtitle
- Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA
- Creators
- E I Golub - Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. golub@biomed.med.yale.eduR C Gupta - Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. golub@biomed.med.yale.eduT Haaf - Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. golub@biomed.med.yale.eduM S Wold - Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. golub@biomed.med.yale.eduC M Radding - Department of Genetics and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. golub@biomed.med.yale.edu
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.26(23), pp.5388-5393
- DOI
- 10.1093/nar/26.23.5388
- PMID
- 9826763
- PMCID
- PMC148005
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Language
- English
- Date published
- 12/01/1998
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024409002771
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