Interaction of long-chain nicotinates with dipalmitoylphosphatidylcholine

Hans-Joachim Lehmler; Azarih Fortis-Santiago; Dhananjaya Nauduri; Paul M Bummer

doi:10.1194/jlr.M400406-JLR200

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Interaction of long-chain nicotinates with dipalmitoylphosphatidylcholine

Journal article

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Interaction of long-chain nicotinates with dipalmitoylphosphatidylcholine

Hans-Joachim Lehmler, Azarih Fortis-Santiago, Dhananjaya Nauduri and Paul M Bummer

Journal of lipid research, Vol.46(3), pp.535-546

03/2005

DOI: 10.1194/jlr.M400406-JLR200

PMID: 15604517

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Abstract

The interaction of four long-chain nicotinates, compounds that are of interest as potential chemopreventive agents, with dipalmitoylphosphatidylcholine (DPPC) was investigated in monolayers at the air-water interface and in fully hydrated bilayers. For the monolayer studies, the compression isotherms of mixtures of the respective nicotinate with DPPC were recorded at various compositions on a hydrochloric acid subphase (pH 1.9-2.1, 37 +/- 2 degrees C). The headgroup of the nicotinates (24-29 A2/molecule) is larger than that of the hydrophobic tail (20 A2/molecule). The pure nicotinates exhibit a temperature- and chain length-dependent transition from an expanded to a condensed phase. Analysis of the concentration dependence of the average molecular area at constant film pressure and the concentration dependence of the breakpoint of the phase transition from the expanded to the condensed state suggests that all four DPPC-nicotinate mixtures are partially miscible at the air-water interface. Although a complex phase behavior with several phase transitions was observed, differential scanning calorimetry studies of the four mixtures are also indicative of the partial miscibility of DPPC and the respective nicotinate. Overall, the complex phase behavior most likely results from the head-tail mismatch of the nicotinates and the geometric packing constraints in the two-component lipid bilayer.

Temperature

Nicotinic Acids - chemistry

Water - chemistry

Calorimetry, Differential Scanning

1,2-Dipalmitoylphosphatidylcholine - chemistry

Molecular Structure

Air

Details

Title: Subtitle: Interaction of long-chain nicotinates with dipalmitoylphosphatidylcholine
Creators: Hans-Joachim Lehmler - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52242, USA. hans-joachim-lehmler@uiowa.edu
Azarih Fortis-Santiago
Dhananjaya Nauduri
Paul M Bummer
Resource Type: Journal article
Publication Details: Journal of lipid research, Vol.46(3), pp.535-546
DOI: 10.1194/jlr.M400406-JLR200
PMID: 15604517
NLM abbreviation: J Lipid Res
ISSN: 0022-2275
eISSN: 1539-7262
Publisher: United States
Grant note: ES 10951 / NIEHS NIH HHS EB 02748 / NIBIB NIH HHS
Language: English
Date published: 03/2005
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute
Record Identifier: 9984000920102771

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