Journal article
Interaction of micropatterned topographical and biochemical cues to direct neurite growth from spiral ganglion neurons
Hearing research, Vol.409, pp.108315-108315
09/15/2021
DOI: 10.1016/j.heares.2021.108315
PMID: 34343850
Abstract
•Photopolymerized topographical methacrylate patterns can be used to microcontact print patterned peptide substrates.•Spiral ganglion neurons align their outgrowth to chemoattractive and chemorepulsive microcontact printed peptide substrates.•These chemoattractive and chemorepulsive cues can be selectively adsorbed onto the ridges or grooves of microtopographical features.•Micropatterning of peptides in cooperative or antagonistic configurations with micro-topographical features enhances or disrupts neurite alignment, respectively.
Functional outcomes with neural prosthetic devices, such as cochlear implants, are limited in part due to physical separation between the stimulating elements and the neurons they stimulate. One strategy to close this gap aims to precisely guide neurite regeneration to position the neurites in closer proximity to electrode arrays. Here, we explore the ability of micropatterned biochemical and topographic guidance cues, singly and in combination, to direct the growth of spiral ganglion neuron (SGN) neurites, the neurons targeted by cochlear implants. Photopolymerization of methacrylate monomers was used to form unidirectional topographical features of ridges and grooves in addition to multidirectional patterns with 90o angle turns. Microcontact printing was also used to create similar uni- and multi-directional patterns of peptides on polymer surfaces. Biochemical cues included peptides that facilitate (laminin, LN) or repel (EphA4-Fc) neurite growth. On flat surfaces, SGN neurites preferentially grew on LN-coated stripes and avoided EphA4-Fc-coated stripes. LN or EphA4-Fc was selectively adsorbed onto the ridges or grooves to test the neurite response to a combination of topographical and biochemical cues. Coating the ridges with EphA4-Fc and grooves with LN lead to enhanced SGN alignment to topographical patterns. Conversely, EphA4-Fc coating on the grooves or LN coating on the ridges tended to disrupt alignment to topographical patterns. SGN neurites respond to combinations of topographical and biochemical cues and surface patterning that leverages both cues enhance guided neurite growth.
Details
- Title: Subtitle
- Interaction of micropatterned topographical and biochemical cues to direct neurite growth from spiral ganglion neurons
- Creators
- Kristy Truong - University of IowaBraden Leigh - University of IowaJoseph T Vecchi - University of IowaReid Bartholomew - University of IowaLinjing Xu - University of IowaC. Allan Guymon - University of IowaMarlan R Hansen - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Hearing research, Vol.409, pp.108315-108315
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.heares.2021.108315
- PMID
- 34343850
- ISSN
- 0378-5955
- eISSN
- 1878-5891
- Grant note
- DOI: 10.13039/100000055, name: National Institute on Deafness and Other Communication Disorders, award: R01 DC012578, T32DC000040; DOI: 10.13039/100006108, name: National Center for Advancing Translational Sciences, award: U54 TR001013; DOI: 10.13039/100000002, name: NIH
- Language
- English
- Date published
- 09/15/2021
- Academic Unit
- Molecular Physiology and Biophysics; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
- Record Identifier
- 9984197255802771
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