Journal article
Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain
The Journal of cell biology, Vol.131(5), pp.1327-1340
12/1995
DOI: 10.1083/jcb.131.5.1327
PMCID: PMC2120645
PMID: 8522593
Abstract
The neuronal microtubule-associated protein tau is required for the development of cell polarity in cultured neurons. Using PC12 cells that stably express tau and tau amino-terminal fragments, we report that tau interacts with the neural plasma membrane through its amino-terminal projection domain. In differentiated PC12 transfectants, tau is found in growth cone-like structures in a nonmicrotubule-dependent manner. In hippocampal neurons, tau is differentially extracted by detergent and enriched in the growth cone and the distal axon when membrane is left intact. In PC12 transfectants, overexpression of tau's amino-terminal fragment, but not of full-length tau, suppresses NGF-induced process formation. Our data suggest that tau's amino-terminal projection domain has an important role in neuritic development and establishes tau as a mediator of microtubule-plasma membrane interactions.
Details
- Title: Subtitle
- Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain
- Creators
- R Brandt - Institute of Neurobiology, University of Heidelberg, GermanyJ LégerG Lee
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.131(5), pp.1327-1340
- Publisher
- United States
- DOI
- 10.1083/jcb.131.5.1327
- PMID
- 8522593
- PMCID
- PMC2120645
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 12/1995
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065378002771
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