Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

M N Usacheva; M C Teichert; Y M Usachev; C E Sievert; M A Biel

doi:10.1002/lsm.20593

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Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

Journal article

Open access

Peer reviewed

Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

M N Usacheva, M C Teichert, Y M Usachev, C E Sievert and M A Biel

Lasers in surgery and medicine, Vol.40(1), pp.55-61

01/2008

DOI: 10.1002/lsm.20593

PMID: 18220267

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Abstract

The difference in photobactericidal efficacy between methylene blue (MB) and toluidine blue (TB) may be explained by their involvement with proteins, lipopolysaccharides (LPS), and siderophores and siderophore-receptor protein complexes on the bacterial outer membrane. This study aims to determine if this is the case by using the fluorescence given off by a pseudomonal siderophore named pyoverdin. Confocal laser scanning microscopy was used to observe the fluorescence of Pseudomonas aeruginosa cells excited at 488 nm in the presence of increasing dye concentrations. Cellular fluorescence at 522 nm progressively decreased with increasing dye concentrations. The Stern-Volmer constants for cellular fluorescence quenching with the dyes were compared to the association constants for dyes complexed with LPS. The quenching of cellular fluorescence was associated with the formation of a ground-state complex between the dyes and pyoverdin-FpvA protein system. MB readily complexed with this system, whereas TB complexed more strongly with LPS. The different affinities of the dyes for both pyoverdin-protein and LPS will affect the contributions of the dyes' interactions with these biopolymers to the overall bacterial photodamage.

Microscopy, Confocal

Tolonium Chloride - metabolism

Oligopeptides

Coloring Agents - pharmacology

Lasers

Methylene Blue - metabolism

Siderophores - radiation effects

Coloring Agents - metabolism

Pseudomonas aeruginosa - radiation effects

Tolonium Chloride - pharmacology

Pseudomonas aeruginosa - drug effects

Methylene Blue - pharmacology

Details

Title: Subtitle: Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells
Creators: M N Usacheva - Advanced Photodynamic Technologies, Inc., Minneapolis, Minnesota 55414, USA
M C Teichert
Y M Usachev
C E Sievert
M A Biel
Resource Type: Journal article
Publication Details: Lasers in surgery and medicine, Vol.40(1), pp.55-61
Publisher: United States
DOI: 10.1002/lsm.20593
PMID: 18220267
ISSN: 0196-8092
eISSN: 1096-9101
Language: English
Date published: 01/2008
Academic Unit: Iowa Neuroscience Institute; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
Record Identifier: 9984040591502771

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