Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells

Vasiliy S Kerov; Michael Natochin; Nikolai O Artemyev

doi:10.1016/j.mcn.2004.10.010

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Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells

Journal article

Peer reviewed

Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells

Vasiliy S Kerov, Michael Natochin and Nikolai O Artemyev

Molecular and cellular neurosciences, Vol.28(3), pp.485-495

03/2005

DOI: 10.1016/j.mcn.2004.10.010

PMID: 15737739

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Abstract

LGN and activator of G-protein signaling 3 (AGS3) belong to the class of G-protein modulators containing G-protein regulatory motifs (GPR proteins). Evidence for the functions of these molecules has only started to emerge. Immunostaining of mouse retina cross-sections and serial tangential sectioning of the retina combined with immunoblot analysis revealed that LGN is expressed in the inner segments of photoreceptor cells. Double immunolabeling demonstrated that, following light-dependent translocation from the outer segments, the alpha-subunit of the visual G-protein transducin (Gtalpha) colocalizes with LGN in the basal part of the inner segments. LGN and Gtalpha coprecipitate from the retinal extracts, supporting the notion of the interaction between the proteins. Furthermore, the GPR domain of LGN potently inhibits receptor-mediated guanine nucleotide exchange and steady-state GTPase activity of transducin. The localization and interaction with Gtalpha suggest LGN roles in modulation of transducin translocation and other photoreceptor cell functions.

Guanine Nucleotides - metabolism

Immunohistochemistry

Mice, Inbred C57BL

Intracellular Signaling Peptides and Proteins

Protein Transport - physiology

Cell Compartmentation - physiology

Carrier Proteins - genetics

Vision, Ocular - physiology

Animals

Carrier Proteins - metabolism

Cattle

Protein Binding

Transducin - metabolism

Mice

Photoreceptor Cells, Vertebrate - metabolism

Protein Structure, Tertiary - physiology

Details

Title: Subtitle: Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells
Creators: Vasiliy S Kerov - Department of Physiology and Biophysics, University of Iowa College of Medicine, BSB 5-532, Iowa City, IA 52242-1109, USA
Michael Natochin
Nikolai O Artemyev
Resource Type: Journal article
Publication Details: Molecular and cellular neurosciences, Vol.28(3), pp.485-495
Publisher: United States
DOI: 10.1016/j.mcn.2004.10.010
PMID: 15737739
ISSN: 1044-7431
eISSN: 1095-9327
Grant note: R01 EY-12682 / NEI NIH HHS
Language: English
Date published: 03/2005
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984025320702771

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