Journal article
Interaction of vitamin C and vitamin E during free radical stress in plasma: An ESR study
Free radical biology & medicine, Vol.14(6), pp.649-653
1993
DOI: 10.1016/0891-5849(93)90146-L
PMID: 8392021
Abstract
To study the interaction of the antioxidant vitamins C and E in a biological system, we used electron spin resonance (ESR) spectroscopy to make serial measurement of ascorbate tocopheroxyl free radicals in plasma subjected to continuous free radical-mediated oxidative stress. Upon initiation of a continuous oxidative stress, we observed an immediate increase in the concentration of ascorbate radical, which reached a peak, and then steadily declined. Only after the virtual disappearance of the ascorbate radical did we observe the appearance of the tocopheroxyl radical. These data are consistent with the hypothesis that ascorbate is the terminal small-molecule antioxidant in biological systems. This is the first experimental demonstration that the predicted thermodynamic hierarchy of ascorbate, α-tocopherol, and their free radicals holds in a biological system containing endogenous levels of these antioxidant vitamins.
Details
- Title: Subtitle
- Interaction of vitamin C and vitamin E during free radical stress in plasma: An ESR study
- Creators
- Mukesh K Sharma - Department of Medicine, The University of Iowa, Iowa City, IA 52242, USAGarry R Buettner - ESR Center, College of Medicine, The University of Iowa, Iowa, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.14(6), pp.649-653
- DOI
- 10.1016/0891-5849(93)90146-L
- PMID
- 8392021
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1993
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047713102771
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