Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice

David H Nichols; Judith E Bouma; Benjamin J Kopecky; Israt Jahan; Kirk W Beisel; David Z Z He; Huizhan Liu; Bernd Fritzsch

doi:10.1007/s00441-019-03163-y

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Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice

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Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice

David H Nichols, Judith E Bouma, Benjamin J Kopecky, Israt Jahan, Kirk W Beisel, David Z Z He, Huizhan Liu and Bernd Fritzsch

Cell and tissue research, Vol.380(3), pp.435-448

06/2020

DOI: 10.1007/s00441-019-03163-y

PMCID: PMC7393901

PMID: 31932950

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https://www.ncbi.nlm.nih.gov/pmc/articles/7393901View

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Abstract

The LIM homeodomain transcription factor Lmx1a shows a dynamic expression in the developing mouse ear that stabilizes in the non-sensory epithelium. Previous work showed that Lmx1a functional null mutants have an additional sensory hair cell patch in the posterior wall of a cochlear duct and have a mix of vestibular and cochlear hair cells in the basal cochlear sensory epithelium. In E13.5 mutants, Sox2-expressing posterior canal crista is continuous with an ectopic "crista sensory epithelium" located in the outer spiral sulcus of the basal cochlear duct. The medial margin of cochlear crista is in contact with the adjacent Sox2-expressing basal cochlear sensory epithelium. By E17.5, this contact has been interrupted by the formation of an intervening non-sensory epithelium, and Atoh1 is expressed in the hair cells of both the cochlear crista and the basal cochlear sensory epithelium. Where cochlear crista was formerly associated with the basal cochlear sensory epithelium, the basal cochlear sensory epithelium lacks an outer hair cell band, and gaps are present in its associated Bmp4 expression. Further apically, where cochlear crista was never present, the cochlear sensory epithelium forms a poorly ordered but complete organ of Corti. We propose that the core prosensory posterior crista is enlarged in the mutant when the absence of Lmx1a expression allows JAG1-NOTCH signaling to propagate into the adjacent epithelium and down the posterior wall of the cochlear duct. We suggest that the cochlear crista propagates in the mutant outer spiral sulcus because it expresses Lmo4 in the absence of Lmx1a.

LIM-Homeodomain Proteins - metabolism

Hair Cells, Auditory, Outer - cytology

Transcription Factors - genetics

LIM Domain Proteins - metabolism

Bone Morphogenetic Protein 4 - metabolism

SOXB1 Transcription Factors - metabolism

Transcription Factors - metabolism

LIM-Homeodomain Proteins - genetics

Animals

Hair Cells, Auditory, Outer - metabolism

Basic Helix-Loop-Helix Transcription Factors - metabolism

Mice, Mutant Strains

Mice

Mutation

Adaptor Proteins, Signal Transducing - metabolism

Details

Title: Subtitle: Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice
Creators: David H Nichols - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
Judith E Bouma - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
Benjamin J Kopecky - Department of Biology, University of Iowa, Iowa City, IA, 52242-1324, USA
Israt Jahan - Department of Biology, University of Iowa, Iowa City, IA, 52242-1324, USA
Kirk W Beisel - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
David Z Z He - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
Huizhan Liu - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
Bernd Fritzsch - Department of Biology, University of Iowa, Iowa City, IA, 52242-1324, USA. bernd-fritzsch@uiowa.edu
Resource Type: Journal article
Publication Details: Cell and tissue research, Vol.380(3), pp.435-448
DOI: 10.1007/s00441-019-03163-y
PMID: 31932950
PMCID: PMC7393901
NLM abbreviation: Cell Tissue Res
ISSN: 0302-766X
eISSN: 1432-0878
Publisher: Germany
Grant note: RO1 AG060504 / National Institutes of Health (US) G20 RR024001 / NCRR NIH HHS P20 GM103471 / NIGMS NIH HHS P01 AG051443 / NIA NIH HHS R01 DC005590 / NIDCD NIH HHS R01 AG060504 / NIA NIH HHS P20 RR018788 / NCRR NIH HHS RO1 DC 005590 / NIH/NIDCD P20 RR 018788 / NCR
Language: English
Date published: 06/2020
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070866502771

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