Interactions of 3T3 fibroblasts and endothelial cells with defined pore features

A K Salem; R Stevens; R G Pearson; M C Davies; S J B Tendler; C J Roberts; P M Williams; K M Shakesheff

doi:10.1002/jbm.10195

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Interactions of 3T3 fibroblasts and endothelial cells with defined pore features

Journal article

Open access

Interactions of 3T3 fibroblasts and endothelial cells with defined pore features

A K Salem, R Stevens, R G Pearson, M C Davies, S J B Tendler, C J Roberts, P M Williams and K M Shakesheff

Journal of biomedical materials research, Vol.61(2), pp.212-217

08/2002

DOI: 10.1002/jbm.10195

PMID: 12007201

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Abstract

The colonization of biodegradable polymer scaffolds with cell populations has been established as the foundation for the engineering of a number of tissues, including cartilage, liver, and bone. Within these scaffolds, the cells encounter a porous environment in which they must migrate across the convoluted polymer surface to generate a homogenous cell distribution. Predicting the interactions between cells and pores is important if scaffold characteristics are to be optimized. Therefore, we investigated the behavior of two model cell types over a range of defined pore features. These pore features range from 5 to 90 microm in diameter and have been fabricated by photolithographic techniques. Quantitatively, the behavior of the cells is dependent on three factors: 1) percentage cell coverage of the surface; 2) pore size; and 3) cell type. Fibroblast cells displayed a co-operative pattern of cell spreading in which pores with diameters greater than the cell dimensions were bridged by groups of cells using their neighbors as supports. Endothelial cells were unable to use neighbors as support structures and failed to bridge pores greater than the cell diameter.

Endothelium, Vascular - cytology

Fibronectins

Tissue Engineering - methods

Actins - metabolism

Focal Adhesions

Coated Materials, Biocompatible

Silicon Compounds

Animals

Cattle

Surface Properties

Fibroblasts - cytology

Mice

Cell Culture Techniques

Porosity

Actins - physiology

3T3 Cells - cytology

Details

Title: Subtitle: Interactions of 3T3 fibroblasts and endothelial cells with defined pore features
Creators: A K Salem - School of Pharmaceutical Sciences, University of Nottingham, Nottingham, NG7 2RD, United Kingdom
R Stevens
R G Pearson
M C Davies
S J B Tendler
C J Roberts
P M Williams
K M Shakesheff
Resource Type: Journal article
Publication Details: Journal of biomedical materials research, Vol.61(2), pp.212-217
DOI: 10.1002/jbm.10195
PMID: 12007201
NLM abbreviation: J Biomed Mater Res
ISSN: 0021-9304
eISSN: 1097-4636
Publisher: United States
Language: English
Date published: 08/2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9983985956002771

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