Journal article
Interactions of intermediate filament protein synemin with dystrophin and utrophin
Biochemical and biophysical research communications, Vol.346(3), pp.768-777
2006
DOI: 10.1016/j.bbrc.2006.05.192
PMID: 16777071
Abstract
Synemin is a unique, very large intermediate filament (IF) protein present in all types of muscle cells, which forms heteropolymeric intermediate filaments (IFs) with the major IF proteins desmin and/or vimentin. We show herein that tissue-purified avian synemin directly interacts with both dystrophin and utrophin, and that specific expressed regions of both of the mammalian (human) synemin isoforms (α-synemin and β-synemin) directly interact with specific expressed domains/regions of the dystrophin and utrophin molecules. Mammalian synemin is also shown to colocalize with dystrophin within muscle cell cultures. These results indicate that synemin is an important IF protein in muscle cells that helps fortify the linkage between the peripheral layer of cellular myofibrils and the costameric regions located along the sarcolemma and the sarcolemma region located within the neuromuscular and myotendinous junctions (NMJs and MTJs).
Details
- Title: Subtitle
- Interactions of intermediate filament protein synemin with dystrophin and utrophin
- Creators
- Rahul C Bhosle - Muscle Biology Group, Departments of Biochemistry, Biophysics, and Molecular Biology and of Animal Science, Iowa State University, Ames, IA 50011-3260, USADaniel E Michele - Howard Hughes Medical Institute, Department of Physiology and Biophysics, Neurology and Internal Medicine, University of Iowa Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242-1101, USAKevin P Campbell - Howard Hughes Medical Institute, Department of Physiology and Biophysics, Neurology and Internal Medicine, University of Iowa Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242-1101, USAZhenlin Li - Pierre and Marie Curie University, UMR7079 CNRS, 75005 Paris, FranceRichard M Robson - Muscle Biology Group, Departments of Biochemistry, Biophysics, and Molecular Biology and of Animal Science, Iowa State University, Ames, IA 50011-3260, USA
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.346(3), pp.768-777
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2006.05.192
- PMID
- 16777071
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 2006
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984020774002771
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