Interassay Comparison of the Tumor Markers CA125, CA15.3, and CA27.29

Gabrielle N Winston-McPherson; Anna E Merrill; Sheng-Ying Lo; Andrew N Hoofnagle; Dina N Greene

doi:10.1373/jalm.2016.021436

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Interassay Comparison of the Tumor Markers CA125, CA15.3, and CA27.29

Journal article

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Peer reviewed

Interassay Comparison of the Tumor Markers CA125, CA15.3, and CA27.29

Gabrielle N Winston-McPherson, Anna E Merrill, Sheng-Ying Lo, Andrew N Hoofnagle and Dina N Greene

The journal of applied laboratory medicine, Vol.2(1), pp.17-24

07/01/2017

DOI: 10.1373/jalm.2016.021436

PMID: 33636961

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https://doi.org/10.1373/jalm.2016.021436View

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Abstract

Cancer antigens 125, 27.29, and 15-3 (CA125, CA27.29, and CA15-3) are markers of ovarian and breast cancer. Comparing tumor marker results across methods is challenging because of the lack of harmonization. Documenting comparability of results is important. Siemens Advia Centaur CA125 and CA27.29 assays were compared to their corresponding Beckman Coulter DxI CA125 and CA15-3 assays. The interassay bias was determined and the manufacturer-recommended reference intervals were evaluated. The DxI CA125 assay demonstrated an overall positive 29% bias relative to the Centaur CA125 assay. The DxI CA15-3 assay demonstrated an overall negative 65% bias relative to the Centaur CA27.29 assay. For patients with multiple comparisons during the study period, the trend of results over time was similar across both sets of assays. Implementing the manufacturer-recommended reference interval for the DxI CA125 assay increased the abnormal flagging rate by 4.5%. In contrast, implementing the manufacturer-recommended reference interval for the DxI CA15-3 assay decreased the abnormal flagging rate by 13.0%. The overall trends for the majority of patients were similar. Therefore, despite the overall biases, transitioning tumor marker assays should not affect clinical interpretation of results.

Details

Title: Subtitle: Interassay Comparison of the Tumor Markers CA125, CA15.3, and CA27.29
Creators: Gabrielle N Winston-McPherson - University of Washington
Anna E Merrill - University of Washington
Sheng-Ying Lo - University of Washington
Andrew N Hoofnagle - University of Washington
Dina N Greene - University of Washington
Resource Type: Journal article
Publication Details: The journal of applied laboratory medicine, Vol.2(1), pp.17-24
DOI: 10.1373/jalm.2016.021436
PMID: 33636961
NLM abbreviation: J Appl Lab Med
ISSN: 2576-9456
eISSN: 2475-7241
Language: English
Date published: 07/01/2017
Academic Unit: Pathology; Injury Prevention Research Center
Record Identifier: 9984186537002771

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