Journal article
Interferon regulatory factor 6 is required for proper wound healing in vivo
Developmental dynamics, Vol.249(4), pp.509-522
04/2020
DOI: 10.1002/dvdy.134
PMCID: PMC9266192
PMID: 31724286
Abstract
Van der Woude syndrome (VWS) is the most common form of syndromic orofacial cleft caused predominantly by mutations in Interferon Regulatory Factor 6 (IRF6). We previously reported that individuals with VWS have increased risk of wound healing complications following cleft repair compared with individuals with nonsyndromic orofacial clefts (nonsyndromic cleft lip and palate-NSCLP). In vitro, absence of IRF6 leads to impaired keratinocyte migration and embryonic wound healing. However, there is currently no data on tissue repair in adult animals and cells with reduced levels of IRF6 like in VWS.
Excisional wounds of Irf6
and wild-type animals were analyzed 4 and 7 days post-wounding. Although all wounds were reepithelialized after 7 days, the epidermal and wound volume of repaired wounds was larger in Irf6
. These data were supported by increased keratinocyte proliferation in the neoformed epidermis and a less mature granulation tissue with increased cytokine levels. This effect was not cell autonomous, as Irf6
neonatal keratinocytes in vitro did not exhibit defects in scratch wound closure or proliferation. Keratinocytes from individuals with VWS also migrated similarly to keratinocytes from NSCLP individuals.
These data support a role for IRF6 in wound healing by regulating keratinocyte proliferation, granulation tissue maturation, and cytokine levels.
Details
- Title: Subtitle
- Interferon regulatory factor 6 is required for proper wound healing in vivo
- Creators
- Lindsey Rhea - University of IowaFranklin J Canady - University of IowaMarc Le - University of IowaTanner Reeb - University of IowaJohn W Canady - University of IowaDeborah S F Kacmarynski - University of IowaRishika Avvari - University of IowaLeah C Biggs - University of IowaMartine Dunnwald - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.249(4), pp.509-522
- DOI
- 10.1002/dvdy.134
- PMID
- 31724286
- PMCID
- PMC9266192
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Grant note
- R37 DE008559 / NIDCR NIH HHS T32 GM008629 / NIGMS NIH HHS R01 AR067739 / NIAMS NIH HHS
- Language
- English
- Date published
- 04/2020
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Craniofacial Anomalies Research Center; Otolaryngology
- Record Identifier
- 9984284328802771
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