Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes

Michael J Lace; James R Anson; Thomas H Haugen; Lubomir P Turek

doi:10.1016/j.virol.2009.12.025

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Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes

Journal article

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Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes

Michael J Lace, James R Anson, Thomas H Haugen and Lubomir P Turek

Virology (New York, N.Y.), Vol.399(2), pp.270-279

2010

DOI: 10.1016/j.virol.2009.12.025

PMID: 20129639

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Abstract

Interferon regulatory factors (IRFs) are critical mediators of gene expression, cell growth and immune responses. We previously demonstrated that interferon (IFN) induction of early viral transcription and replication in several mucosal HPVs requires IRF-1 binding to a conserved interferon response element (IRE). Here we show that the IRF-2 protein serves as a baseline transactivator of the HPV-16 major early promoter, P97. Cotransfections in IRF knockout cells confirmed that basal HPV-16 promoter activity was supported by both IRF-1 and IRF-2 complexes interacting with the promoter-proximal IRE in a dose-dependent manner. Furthermore, HPV-16 E7 expression downregulates the IRF-2 promoter, thus linking IRF-2 levels to viral transforming gene expression through a negative feedback mechanism. Taken together, these observations reveal a complex viral strategy utilizing multiple signal transduction pathways during the establishment and maintenance of HPV persistence.

Papillomavirus

Signal transduction

Immunoevasion

Cytokine induction

Keratinocyte

IRF

Gene expression

Details

Title: Subtitle: Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes
Creators: Michael J Lace - Department of Pathology, VAMC, University of Iowa, Iowa City, IA 52242, USA
James R Anson - Department of Pathology, VAMC, University of Iowa, Iowa City, IA 52242, USA
Thomas H Haugen - Department of Pathology, VAMC, University of Iowa, Iowa City, IA 52242, USA
Lubomir P Turek - Department of Pathology, VAMC, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.399(2), pp.270-279
DOI: 10.1016/j.virol.2009.12.025
PMID: 20129639
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2010
Academic Unit: Pathology
Record Identifier: 9984047642002771

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