Journal article
Intergenic regions between tandem gp63 genes influence the differential expression of gp63 RNAs in Leishmania chagasi promastigotes
The Journal of biological chemistry, Vol.270(20), pp.12133-12139
05/19/1995
DOI: 10.1074/jbc.270.20.12133
PMID: 7744862
Abstract
The major surface protease, gp63, of Leishmania chagasi is encoded by 18 or more tandem msp genes that can be grouped into three classes on the basis of their unique 3'-untranslated sequences (3'-UTRs) and their differential expression. RNAs from the mspLs occur predominantly during the logarithmic phase of promastigote growth in vitro, RNAs from the mspSs are present mainly in stationary phase, and RNAs from mspCs occur throughout growth in culture. All three classes of gp63 genes are constitutively transcribed during all growth phases, indicating that their expression is post-transcriptionally regulated. Chimeric plasmids containing the three different 3'-UTRs and downstream intergenic regions (IRs) fused downstream of the beta-galactosidase (beta-gal) coding region were transfected into L. chagasi, and their effects on beta-gal RNA processing and enzymatic activity were examined. The presence of the 3'-UTRs by themselves had no substantive effect on beta-gal expression. However, the 3'-UTR from a mspS plus its IR resulted in about 20-fold more beta-gal activity and RNA in stationary phase relative to logarithmic phase cells. In contrast, the 3'-UTRs plus IRs of mspL and mspC had either no or little effect, respectively, on beta-gal expression. Thus, differential expression of the mspLs and mspSs is post-transcriptionally controlled by different mechanisms.
Details
- Title: Subtitle
- Intergenic regions between tandem gp63 genes influence the differential expression of gp63 RNAs in Leishmania chagasi promastigotes
- Creators
- Ramesh Ramamoorthy - Department of Biochemistry, University of Iowa, Iowa City 52242, USAKristin G SwihartJames J McCoyMary E WilsonJohn E Donelson
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.270(20), pp.12133-12139
- DOI
- 10.1074/jbc.270.20.12133
- PMID
- 7744862
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- United States
- Grant note
- AI30126 / NIAID NIH HHS AI32135 / NIAID NIH HHS
- Language
- English
- Date published
- 05/19/1995
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984001123902771
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