Journal article
Interlaboratory evaluation of endotoxin analyses in agricultural dusts--comparison of LAL assay and mass spectrometry
Journal of Environmental Monitoring, Vol.7(12), pp.1371-1377
10/11/2005
DOI: 10.1039/b509256f
PMID: 16307099
Abstract
<p>Endotoxin exposure is associated with wheeze and asthma morbidity, while early life exposure may reduce risk of allergy and asthma. Unfortunately, it is difficult to compare endotoxin results from different laboratories and environments. We undertook this study to determine if lipopolysaccharide (LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for Limulus assay of endotoxin. Lyophilized aliquots of filter extracts were analyzed for 3-hydroxy fatty acids (3-OHFAs) as a marker of LPS using gas chromatography-mass spectrometry. There were significant differences in endotoxin assay and GC-MS (LPS) results between laboratories for all dust types (p < 0.01). Patterns of differences between labs varied by dust type. Relationships between assay and GC/MS results also depended on dust type. The percentages of individual 3-OHFA chain lengths varied across labs (p < 0.0001) suggesting that each lab recovered a different fraction of the LPS available. The presence of large amounts of particle associated LPS and absence of a freezing thawing cycle were associated with lower correlations between LPS and bioactivity, consistent with an absence of Limulus response to cell-bound endotoxin. These data suggest that extraction methods affect endotoxin measurements. The LAL methods may be most suitable when comparing exposures within similar environments; GC-MS offers additional information helpful in optimizing sample treatment and extraction. GC-MS may be of use when comparing across heterogeneous environments and should be considered for inclusion in future studies of human health outcomes.</p>
Details
- Title: Subtitle
- Interlaboratory evaluation of endotoxin analyses in agricultural dusts--comparison of LAL assay and mass spectrometry
- Creators
- Stephen J. Reynolds - Colorado State University - Fort CollinsDonald K. Milton - Harvard UniversityDick Heederik - University of UtrechtPeter S. Thorne - University of IowaKelley J. Donham - NIOSHElizabeth A. Croteau - University of IowaKevin M. Kelly - University of IowaJeroen Douwes - University of UtrechtDaniel Lewis - NIOSHMike Whitmer - NIOSHIan Connaughton - Bendigo Agricultural CentreSharon Koch - Bendigo Agricultural CentrePer Malmberg - National Institute for Working LifeBritt-Marie Larsson - National Institute for Working LifeJim Deddens - University of CincinnatiAnita Saraf - University of LundLennart Larsson - University of Lund
- Resource Type
- Journal article
- Publication Details
- Journal of Environmental Monitoring, Vol.7(12), pp.1371-1377
- DOI
- 10.1039/b509256f
- PMID
- 16307099
- NLM abbreviation
- J Environ Monit
- ISSN
- 1464-0325
- eISSN
- 1464-0333
- Publisher
- England
- Grant note
- U07 CCU 706145-05 / ODCDC CDC HHS P30 ES05605 / NIEHS NIH HHS U50 OH008085 / NIOSH CDC HHS R01 OH03489 / NIOSH CDC HHS P30 ES00002 / NIEHS NIH HHS R01 ES-07036 / NIEHS NIH HHS
- Language
- English
- Date published
- 10/11/2005
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9983557799602771
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