Journal article
Interleukin-10 Modulates the Severity of Hypersensitivity Pneumonitis in Mice
American journal of respiratory cell and molecular biology, Vol.19(5), pp.812-818
11/1998
DOI: 10.1165/ajrcmb.19.5.3153
PMID: 9806746
Abstract
Hypersensitivity pneumonitis (HP) is an inflammatory lung disease characterized by granuloma formation. We recently showed that interferon-gamma (IFN-gamma) is essential for inflammation and granuloma formation in HP. Interleukin-10 (IL-10) counteracts many of the biologic effects of IFN-gamma, suggesting that IL-10 modulates inflammation and granuloma formation in HP. We compared the expression of HP in C57BL/6 mice that lack IL-10 (IL-10 knockout [KO]) with that in wild-type (WT) littermates. IL-10 KO and WT mice were exposed to the thermophilic bacteria Saccharopolyspora rectivirgula or to saline alone for 3 wk. The IL-10 KO mice had higher cell counts in their bronchoalveolar lavage fluid (2.85 +/- 0. 43 x 10(6)) than did WT mice (1.4 +/- 0.3 x 10(6)/ml; P < 0.03), with a more prominent neutrophil response. They also had greater inflammation after antigen exposure than did the WT mice (P < 0. 0001). There was increased upregulation of IFN-gamma, IL-1, and tumor necrosis factor-alpha (TNF-alpha) mRNAs in the lungs of IL-10 KO mice. Adenovirus-mediated gene transfer of IL-10 to the liver of IL-10 KO mice reduced the inflammation from that seen in WT mice. These studies show that IL-10 has important anti-inflammatory properties in HP, and that lack of this cytokine leads to a more severe granulomatous inflammatory response.
Details
- Title: Subtitle
- Interleukin-10 Modulates the Severity of Hypersensitivity Pneumonitis in Mice
- Creators
- Gunnar GudmundssonAssumpció BoschBeverly L DavidsonDaniel J BergGary W Hunninghake
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.19(5), pp.812-818
- DOI
- 10.1165/ajrcmb.19.5.3153
- PMID
- 9806746
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Language
- English
- Date published
- 11/1998
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094214602771
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