Journal article
Interleukin-10 protects against aging-induced endothelial dysfunction
Physiological reports, Vol.1(6), pp.1-n/a
11/01/2013
DOI: 10.1002/phy2.149
PMCID: PMC3871464
PMID: 24400151
Abstract
Carotid and cerebrovascular disease increase markedly with age contributing to stroke and cognitive impairment. Inflammation is a key element of vascular disease. In these studies, we tested the hypothesis that interleukin-10 (IL-10), a potent anti-inflammatory cytokine, protects against aging-induced endothelial dysfunction. Responses of carotid arteries from adult (5 ± 1 months) and old (22 ± 1 months) wild-type and IL-10-deficient mice were examined in vitro. Acetylcholine (an endothelium-dependent agonist) produced relaxation in arteries from adult wild-type that was not altered in old mice. In contrast, relaxation to acetylcholine in arteries from old IL-10-deficient mice was reduced by ∼50% (
P
< 0.05). Tempol, a scavenger of superoxide, did not affect responses in adult or old wild-type mice, but restored vasodilation to acetylcholine to normal in old IL-10-deficient mice. Responses of the carotid artery to nitroprusside (an endothelium-independent agonist) were not altered in any group. Vascular expression of IL-6 (a proinflammatory mediator of vascular disease) and components of NADPH oxidase (a major source of superoxide) was increased in old IL-10-deficient mice compared with wild-type (
P
< 0.05). These findings provide the first evidence that age-related and superoxide-mediated endothelial dysfunction occurs earlier with IL-10 deficiency. Our findings suggest a novel role for IL-10 to protect against age-related increases in expression of IL-6, oxidative stress, and endothelial dysfunction.
Details
- Title: Subtitle
- Interleukin-10 protects against aging-induced endothelial dysfunction
- Creators
- Dale A Kinzenbaw - University of IowaYi Chu - University of IowaRicardo A Peña Silva - University of IowaSean P Didion - Roy J. and Lucille A. Carver College of MedicineFrank M Faraci - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Physiological reports, Vol.1(6), pp.1-n/a
- DOI
- 10.1002/phy2.149
- PMID
- 24400151
- PMCID
- PMC3871464
- NLM abbreviation
- Physiol Rep
- ISSN
- 2051-817X
- eISSN
- 2051-817X
- Publisher
- Blackwell Publishing Ltd
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: HL-38901, NS-24621, HL-62984, HL-089884, HL-107632; DOI: 10.13039/100000968, name: American Heart Association, award: 0575092N
- Language
- English
- Date published
- 11/01/2013
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Neurosurgery; Internal Medicine
- Record Identifier
- 9984303853902771
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