Journal article
Interleukin 2 inhibits in vitro granulocyte-macrophage colony formation
The Journal of immunology (1950), Vol.139(6), pp.1880-1884
1987
DOI: 10.4049/jimmunol.139.6.1880
PMID: 2442258
Abstract
We have previously shown that murine bone marrow cells cultured with interleukin 2 (IL-2) produce interferon-alpha/beta (MuIFN-alpha/beta) and that IFN-alpha/beta can suppress in vitro granulocyte-macrophage colony-forming cell formation (GM-CFC). In this study, IL-2 was directly assessed for its ability to inhibit in vitro granulocyte and/or macrophage colony-forming cell formation (GM-CFC/M-CFC). C57BL/6 bone marrow cells were cultured with different colony-stimulating factors (CSF), i.e., partially purified macrophage-CSF (M-CSF) or recombinant granulocyte and macrophage CSF (GM-CSF) in the presence or absence of different IL-2 preparations. Partially purified mouse IL-2 or recombinant human or mouse IL-2 (rHuIL-2 and rMuIL-2) totally inhibit GM-CFC and M-CFC formation at 7 days of culture. The level of inhibition mediated by IL-2 was concentration-dependent, with as little as 1 U/ml giving total inhibition of colony formation. The ability of IL-2 to inhibit colony formation was completely abolished by treatment with antisera to IL-2. MuIFN-alpha/beta and MuIFN-gamma appeared to play no role in IL-2-induced myelo-suppression in that addition of antisera to these IFN failed to block IL-2-induced suppression. Myelo-suppression mediated by IL-2 was independent of the concentration of CSF used in the bone marrow cultures. Suppression was also not dependent upon the initial presence of T cells or natural killer (NK) cells. Bone marrow cells depleted of Thy-1+, Lyt-1+, Lyt-2+, NK-1.1+, Asialo GM1+, or Qa-5+ cells were as susceptible to IL-2 induced suppression as untreated or complement-treated bone marrow cells. These results suggest that IL-2 may play an important role in regulating different aspects of hematopoiesis.
Details
- Title: Subtitle
- Interleukin 2 inhibits in vitro granulocyte-macrophage colony formation
- Creators
- Antonella Naldini - Univ. Siena, inst. gen. physiology, Siena, ItalyW. Robert Fleischmann Jr - Univ. Siena, inst. gen. physiology, Siena, ItalyZuhair K Ballas - Univ. Siena, inst. gen. physiology, Siena, ItalyKathleen D Klimpel - Univ. Siena, inst. gen. physiology, Siena, ItalyGary R Klimpel - Univ. Siena, inst. gen. physiology, Siena, Italy
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.139(6), pp.1880-1884
- Publisher
- American Association of Immunologists
- DOI
- 10.4049/jimmunol.139.6.1880
- PMID
- 2442258
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1987
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094656502771
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