Journal article
Interleukin-3 signals through multiple isoforms of Stat5
The EMBO journal, Vol.14(7), pp.1402-1411
04/03/1995
DOI: 10.1002/j.1460-2075.1995.tb07126.x
PMCID: PMC398225
PMID: 7537213
Abstract
The interleukin (IL)-3 family of cytokines mediates its numerous effects on myeloid growth and maturation by binding a family of related receptors. It has been shown recently that IL-3 induces the activation of two distinct cytoplasmic signal transducing factors (STFs) that are likely to mediate the induction of immediate early genes. In immature myeloid cells, IL-3 activates STF-IL-3a, which comprises two tyrosine-phosphorylated DNA binding proteins of 77 and 80 kDa. In mature myeloid cells, IL-3 and granulocyte-macrophage colony-stimulating factor activate STF-IL-3b, which consists of a 94 and 96 kDa tyrosine-phosphorylated DNA binding protein. Peptide sequence data obtained from the purified 77 and 80 kDa proteins (p77 and p80) indicate that they are closely related but are encoded by distinct genes. Both peptide and nucleotide sequence data demonstrate that these two proteins are the murine homologs of ovine mammary gland factor (MGF)/Stat5. The peptide data also indicate that p77 and p80 are phosphorylated on tyrosine 699, a position analogous to the tyrosine that is phosphorylated in Stat1 and Stat2 in response to interferon. Additionally, antiserum raised against bacterially expressed p77/p80 recognizes the 94 and 96 kDa protein components of STF-IL-3b, suggesting that these may be additional isoforms of Stat5. These studies indicate that the IL-3 family of ligands is able to activate multiple isoforms of the signal transducing protein Stat5.
Details
- Title: Subtitle
- Interleukin-3 signals through multiple isoforms of Stat5
- Creators
- M Azam - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAHediye Erdjument-Bromage - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USABrendt Kreider - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAM Xia - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAF Quelle - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAR Basu - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAC Saris - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAPaul Tempst - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAJames N Ihle - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USAChristian Schindler - Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.14(7), pp.1402-1411
- DOI
- 10.1002/j.1460-2075.1995.tb07126.x
- PMID
- 7537213
- PMCID
- PMC398225
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Language
- English
- Date published
- 04/03/1995
- Academic Unit
- Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040535502771
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