Journal article
Interleukin‐1β‐induced Prostaglandin E2 Production in Human Myometrial Cells: Role of a Pertussis Toxin‐sensitive Component
American journal of reproductive immunology (1989), Vol.45(3), pp.142-147
03/2001
DOI: 10.1111/j.8755-8920.2001.450304.x
Abstract
Problem: The objective of this study was to evaluate the possible role of pertussis toxin (PTX)-sensitive G-protein(s) in interleukin-1beta (IL-1) signaling in human myometrial cells (HMC).
Method: Primary cultures of HMC were stimulated with human recombinant IL-1 alone or in combination with PTX. Prostaglandin (PG) E2 in the medium was measured by radioimmunoassay, cyclooxygenase type 2 (Cox-2) and IkappaB by western analysis, and the activities of two members of the mitogen-activated protein kinase (MAPK) family of enzymes, ERK-2 and JNK, by the phosphorylation of appropriate substrates.
Results: IL-1 increased PGE2 output during an 18-hr long incubation by 21.7-fold (n = 5 experiments). This increase was inhibited by 57% after pretreatment overnight with PTX. IL-1-induced expression of Cox-2 protein was also suppressed to a similar degree in PTX-treated HMC cultures. Degradation of the nuclear factor kappa B (NF-kappaB)-inhibiting protein (IkappaB), a critical step in IL-1 signaling to the nucleus, was significantly inhibited by PTX, as was IL-1-induced activation of ERK-2 and JNK.
Conclusions: It is suggested that the occupied IL-1 receptor-generated signal in HMC is transmitted by multiple pathways. One is coupled to a PTX-sensitive G-protein upstream from the MAPK phosphorylation cascade. This, in turn, may interact with another signaling pathway, the activation of NF-kappaB, via the phosphorylation of the IkappaB kinase complex.
Details
- Title: Subtitle
- Interleukin‐1β‐induced Prostaglandin E2 Production in Human Myometrial Cells: Role of a Pertussis Toxin‐sensitive Component
- Creators
- FRANK Hertelendy - Saint Louis UniversityPRERNA RastogiMIKLÓS MolnárROBERTO Romero
- Resource Type
- Journal article
- Publication Details
- American journal of reproductive immunology (1989), Vol.45(3), pp.142-147
- Publisher
- Munksgaard
- DOI
- 10.1111/j.8755-8920.2001.450304.x
- ISSN
- 1046-7408
- eISSN
- 1600-0897
- Number of pages
- 6
- Language
- English
- Date published
- 03/2001
- Academic Unit
- Pathology
- Record Identifier
- 9984186779202771
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