Journal article
Internal and surface subpopulations of the major surface protease (MSP) of Leishmania chagasi
Molecular and biochemical parasitology, Vol.139(2), pp.173-183
2005
DOI: 10.1016/j.molbiopara.2004.11.005
PMID: 15664652
Abstract
Major surface protease (MSP) facilitates
Leishmania promastigote evasion of complement-mediated lysis in the mammalian host and enhances host macrophage phagocytosis of the promastigotes. We previously showed that the steady-state abundance of MSP protein increases 14-fold during in vitro cultivation of
L. chagasi promastigotes from logarithmic to stationary phase, despite the fact that the total amount of
MSP mRNA does not increase. Furthermore, 10 major MSP isoforms are differentially expressed in different promastigote growth phases, and attenuation of parasites by long-term in vitro cultivation influences MSP isoform expression. Herein, we report that although about two-thirds of newly synthesized MSP becomes surface localized, the rest of the MSP does not reach the promastigote surface. This internal MSP is stable without detectable decrease in abundance up to 6 days after biosynthesis. Furthermore, surface-localized MSP is released at different rates from logarithmic and stationary phase virulent
Leishmania promastigotes. These data are consistent with the hypothesis that the major mechanism regulating MSP abundance is the rate of loss of surface-localized MSP from the promastigote surface, and that internally localized MSP is very stable.
Details
- Title: Subtitle
- Internal and surface subpopulations of the major surface protease (MSP) of Leishmania chagasi
- Creators
- Chaoqun Yao - VA Medical Center, University of Iowa, Iowa City, IA 52242, USAJiwen Luo - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAChristine Hsiao - Program in Molecular Biology, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMary E Wilson - VA Medical Center, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.139(2), pp.173-183
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.molbiopara.2004.11.005
- PMID
- 15664652
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 2005
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984001140002771
Metrics
26 Record Views