Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

Dominique H Limoli; Elizabeth A Warren; Kaitlin D Yarrington; Niles P Donegan; Ambrose L Cheung; George A O'Toole

doi:10.7554/eLife.47365

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Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

Journal article

Open access

Peer reviewed

Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa

Dominique H Limoli, Elizabeth A Warren, Kaitlin D Yarrington, Niles P Donegan, Ambrose L Cheung and George A O'Toole

eLife, Vol.8, e47365

11/12/2019

DOI: 10.7554/eLife.47365

PMCID: PMC6910820

PMID: 31713513

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https://doi.org/10.7554/eLife.47365View

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Abstract

Microbes often live in multispecies communities where interactions among community members impact both the individual constituents and the surrounding environment. Here, we developed a system to visualize interspecies behaviors at initial encounters. By imaging two prevalent pathogens known to be coisolated from chronic illnesses, and , we observed can modify surface motility in response to secreted factors from . Upon sensing transitioned from collective to single-cell motility with an associated increase in speed and directedness - a behavior we refer to as 'exploratory motility'. Explorer cells moved preferentially towards and invaded colonies through the action of the type IV pili. These studies reveal previously undescribed motility behaviors and lend insight into how senses and responds to other species. Identifying strategies to harness these interactions may open avenues for new antimicrobial strategies.

Bacterial Physiological Phenomena

Cystic Fibrosis - microbiology

Fimbriae, Bacterial - physiology

Humans

Microbial Interactions

Movement - physiology

Pseudomonas aeruginosa - physiology

Pseudomonas Infections - microbiology

Species Specificity

Staphylococcal Infections - microbiology

Staphylococcus aureus - metabolism

Staphylococcus aureus - physiology

Details

Title: Subtitle: Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa
Creators: Dominique H Limoli - Roy J. and Lucille A. Carver College of Medicine
Elizabeth A Warren - University of Iowa
Kaitlin D Yarrington - Roy J. and Lucille A. Carver College of Medicine
Niles P Donegan - Dartmouth College
Ambrose L Cheung - Dartmouth College
George A O'Toole - Dartmouth College
Resource Type: Journal article
Publication Details: eLife, Vol.8, e47365
DOI: 10.7554/eLife.47365
PMID: 31713513
PMCID: PMC6910820
NLM abbreviation: Elife
ISSN: 2050-084X
eISSN: 2050-084X
Grant note: Grant R37 AI83256 / NIH HHS R37 AI083256 / NIAID NIH HHS CFF Postdoc-to-Faculty Transition Award LIMOLI18F5 / Cystic Fibrosis Foundation Postdoctoral Fellowship LIMOLI15F0 / Cystic Fibrosis Foundation
Language: English
Date published: 11/12/2019
Academic Unit: Microbiology and Immunology
Record Identifier: 9984297431302771

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