Journal article
Intersubject Variation in ACE2 Protein Expression in Human Airway Epithelia and Its Relationship to Severe Acute Respiratory Syndrome Coronavirus 2
The Journal of infectious diseases, Vol.224(8), pp.1357-1361
10/15/2021
DOI: 10.1093/infdis/jiab383
PMCID: PMC8557670
PMID: 34289058
Abstract
Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 ) initiates entry into airway epithelia by binding its receptor, angiotensin-converting enzyme 2 (ACE2).
Methods. To explore whether interindividual variation in ACE2 abundance contributes to variability in coronavirus disease 2019 (COVID-19) outcomes, we measured ACE2 protein abundance in primary airway epithelial cultures derived from 58 human donor lungs.
Results. We found no evidence for sex- or age-dependent differences in ACE2 protein expression. Furthermore, we found that variations in ACE2 abundance had minimal effects on viral replication and induction of the interferon response in airway epithelia infected with SARS-CoV-2.
Conclusions. Our results highlight the relative importance of additional host factors, beyond viral receptor expression, in determining COVID-19 lung disease outcomes.
Details
- Title: Subtitle
- Intersubject Variation in ACE2 Protein Expression in Human Airway Epithelia and Its Relationship to Severe Acute Respiratory Syndrome Coronavirus 2
- Creators
- Kun Li - University of IowaChristine Wohlford-Lenane - University of IowaJennifer A. Bartlett - University of IowaPaul B. McCray - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.224(8), pp.1357-1361
- Publisher
- Oxford Univ Press
- DOI
- 10.1093/infdis/jiab383
- PMID
- 34289058
- PMCID
- PMC8557670
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Number of pages
- 5
- Grant note
- Cell Morphology Core and Pathology Core P01 AI060699 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30 DK054759 / Center for Gene Therapy for Cystic Fibrosis (National Institutes of Health) Cystic Fibrosis Foundation; Italian Cystic Fibrosis Research Foundation Roy J. Carver Charitable Trust
- Language
- English
- Date published
- 10/15/2021
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984297330102771
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