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Intracellular delivery of RNA-based therapeutics using aptamers
Journal article   Peer reviewed

Intracellular delivery of RNA-based therapeutics using aptamers

Kristina W. Thiel and Paloma H. Giangrande
Therapeutic delivery, Vol.1(6), pp.849-861
12/01/2010
DOI: 10.4155/TDE.10.61
PMCID: PMC3106310
PMID: 21643487
url
https://www.ncbi.nlm.nih.gov/pmc/articles/3106310View
Open Access

Abstract

The clinical potential of siRNAs for silencing genes critical to disease progression is clear, but a fail-proof method for delivering siRNAs to the cytoplasm of diseased tissues or cells has yet to be identified. A variety of delivery approaches have been explored to directly or indirectly couple siRNAs to delivery vehicles. This review explores the use of synthetic single-stranded DNA and RNA aptamers as a means to deliver siRNAs, shRNAs and antisense oligonucleotides for therapeutic intervention. Topics covered include: the advantages and challenges of using aptamers as delivery tools; current aptamer-mediated siRNA delivery platforms for the treatment of cancer and HIV; and emerging methodologies for the identification of aptamers capable of internalizing into target cell types.
Life Sciences & Biomedicine Pharmacology & Pharmacy Science & Technology

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